Nucleic acids encoding modified olfactory cyclic nucleotide...

Details Nucleic acids encoding modified olfactory cyclic nucleotide... Nucleic acids encoding modified olfactory cyclic nucleotide...

2007-01-23

2007-01-23

Saoud, Christine J. (Department: 1647)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S252300, C435S320100, C530S350000, C536S023500

Reexamination Certificate

active

10295573

ABSTRACT:
The present invention provides modified cyclic nucleotide gated (CNG) channels. In particularly preferred embodiments, the modified CNG channels exhibit increased sensitivity and specificity for cAMP, as compared to wild-type CNG channels. In additional embodiments, regulation by Ca2+-calmodulin has been removed in the modified CNG channels. Convenient optical methods for detecting changes in cAMP, taking advantage of the Ca2+permeability of the channel are also provided by the present invention. In addition, electrophysiological methods are further provided.

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