Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2007-10-09
2007-10-09
Mehta, Ashwin (Department: 1638)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S320100, C435S419000, C536S023720, C800S305000, C800S278000
Reexamination Certificate
active
10276968
ABSTRACT:
The coat protein of lettuce big-vein virus (LBVV) was purified from highly purified LBVV, and its partial amino acid sequences were determined. An RNA encoding the coat protein of LBVV was cloned by polymerase chain reaction using primers designed based on the determined amino acid sequences information, and its primary structure was elucidated. Moreover, the present inventors succeeded not only in isolating RNA molecules of a plurality of LBVV-encoded proteins, including LBVV polymerase, by carrying out 3′RACE and 5′RACE using primers designed based on the resulting sequence information, but also in determining their primary structure. It was found that the use of these made it possible to produce lettuce resistant to LBVV and to diagnose infections with LBVV.
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English translation of Sasaya, T. et al., “Amino Acid Sequence of a Protein Encoded in RNA1 of Lettuce Big-vein Virus,” presented at The Annual Meeting of the Phytopathological Society of Japan, Abstract No. 2-29, p. 55, Sendai, Japan (Apr. 2-4, 2001).
English translation of Sasaya, T. et al., “Nucleotide Sequence of the Coat Protein Gene of Lettuce Big-vein Virus,”Jpn. J. Phytopathol. 66:298, Abstract No. 64, The Phytopathological Society of Japan (Dec. 2000).
English translation of Sasaya, T. et al., “Nucleotide Sequence of the Coat Protein of Lettuce Big-vein Virus,” in the Drafts of Summaries of Speech at the Meeting of the Kansai Division of Phytopathological Society of Japan, p. 74, Abstract No. 64, Kensai, Japan (Oct. 12-13, 2000).
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Koganezawa Hiroki
Sasaya Takahide
Mehta Ashwin
National Agricultural Research Organization
Sterne Kessler Goldstein & Fox PLLC
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