Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2007-07-31
2007-07-31
Kruse, David H. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C435S320100, C435S419000, C536S023600, C800S278000
Reexamination Certificate
active
10482951
ABSTRACT:
Provided are nucleic acid sequences fromBrassica oleraceaplants that encode enzymes involved in the synthesis of a glucosinolate molecule, including BoGSL-ALK, and BoGSL-ELONG, and methods of their use. The sequences find particular use in modifying the glucosinolate content of a plant. Also provided are primers for these two genes and a third key gene in the glucosinolate pathway, BoGSL-PRO, that can be used for developing molecular markers for assisted selection of plants with specific glucosinolate compositions. Additionally, compositions and methods for a simple, reliable and efficient PCR-based marker system, named sequence-related amplification polymorphism (SRAP), that finds use in the identification of coding sequences in the genome of a plant are provided.
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Li Genyi
Quiros Carlos
Kruse David H.
Kumar Vinod
Regents of the University of California
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