Nucleic acids encoding BSSL variants

Details Nucleic acids encoding BSSL variants Nucleic acids encoding BSSL variants

1994-03-01

1998-10-27

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 435 701, 435 703, 435 711, 4351723, 435200, 435243, 4353201, 435325, 536 231, 536 232, 536 235, 935 66, C07H 2104, C12N 500, C12N 1512, C12P 2100

Patent

active

058276831

ABSTRACT:
The invention discloses nucleic acids encoding variant Bile Salt Stimulated Lipase (BSSL; EC 3.1.1.1). The encoded variant BSSL enzymes maintain catalytic activity but contain fewer glycosylation sites that full-length BSSL. This reduced glycosylation facilitates purification and characterization of recombinant BSSL proteins.

REFERENCES:
patent: 5200183 (1993-04-01), Tang et al.
Blackberg, Eur. J. Biochem., 192, pp. 543-550, (1990).
Baba et al. "Structure of Human . . ." Biochemistry 30 (1991) 500-510.
DiPersio et al. "Identification of the Active Site" J. Bio. Chemistry 265 (1990) 16801-16806.
DiPersio et al., "Site-specific mutagenesis of an essential histidine residue . . . " J. Biol. Chemistry 266 (1991) 4033-6.
DiPersio et al. "Apartic acid 320 is required . . . " J. Bio. Chemistry R68 (1993) 300-304.
Rieger et al., EDS. in "Glossary of Genetics and Cytogenetics" Springer-Verlag, Berlin, 1976, p. 282.

Affiliated with

Also associated with

Rating

Nucleic acids encoding BSSL variants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acids encoding BSSL variants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acids encoding BSSL variants will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1613021