Nucleic acids encoding ascorbate free radial reductase and their

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

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800DIG44, 4351723, 435189, 536 236, 536 241, A01H 500, C12N 1529, C12N 1584, C12N 1582, C12N 1553

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active

057508720

ABSTRACT:
The present invention provides isolated nucleic acid constructs comprising tomato AFR reductase polynucleotide sequences. The polynucleotides encode AFR reductase polypeptides having ability to catalyze the reduction of AFR, which provide enhanced resistance to oxidative stress. The invention further provides transgenic plants comprising the recombinant expression cassettes, as well as methods of enhancing resistance to oxidative stress in a plant.

REFERENCES:
Sano, Satoshi, et al. (1994) "cDNA Cloning of Monodehydroascorbate Radical Reductase from Cucumber: a High Degree of Homology in Terms of Amino Acid Sequence between This Enzyme and Bacterial Flavoenzymes", Plant Cell Physiol., 35(3): 425-437.
Trumper, Susanne, et al. (1994) "A novel dehydroascorbate reductase from spinach chloroplasts homologous to plant trypsin inhibitor", FEBS Letters 352:159-162.
Murthy, Siva S., et al. (1994) "Molecular Cloning and Characterization of cDNA Encoding Pea Monodehydroascorbate Reductase", The Journal of Biological Chemistry, 269(49):31129-31133.
Hossain, M. Anwar, et al. (1985) "Monodehydroascorbate Reductase from Cucumber Is a Flavin Adenine Dinucleotide Enzyme", The Journal of Biological Chemistry, 260(24): 12920-12926.
Wells, William W., et al. (1993) "Thioltransferases", Adv. in Enzym. Related Areas Mol. Biol., 66:149-201.
Napoli et al. 1990. Plant Cell 2: 279-289.
Smith et al. 1988. Nature 334: 724-726.
De Tullio et al. 1992. Boll. Soc. It. Biol. Sper. 68(10): 613-617.

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