Nucleic acids encoding antifungal polypeptides and uses thereof

Details Nucleic acids encoding antifungal polypeptides and uses thereof Nucleic acids encoding antifungal polypeptides and uses thereof

2009-07-16

2010-11-02

Ibrahim, Medina A (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide confers pathogen or pest resistance

C800S278000, C800S298000, C800S295000, C800S317000, C800S320000, C435S320100, C435S468000, C435S419000

Reexamination Certificate

active

07825294

ABSTRACT:
Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

REFERENCES:
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patent: WO 2006/014316 (2006-02-01), None
Rey, M, et al., “Unexpected Homology Between Inducible Cell Wall Protein QID74 of Filamentous Fungi and BR3 Salivary Protein of the InsectChironomus,” Proc. Natl. Acad. Sci. USA, 1998, pp. 6212-6216, vol. 95.
The USPTO Publication Site for Issued and Published Sequences (PSIPS), XP-002442732, SEQ ID No. 346842 for US 20040214272.
Database EMBL, Jan. 2, 2004, EBI Accession No. EMBL:CF453104, “wt—2—018 fusarium verticillioides wild type subtraction libraryGibberella moniliformiscDNA, mRNA sequence”, XP002442733.
Database EMBL, Jul. 12, 2001, EBI Accession No. EMBL:BI200659, “olallfs.rlFusarium sporotrichioidesTri 10 overexpressed cDNA libraryFusarium sporotrichioidescDNA clone olallfs 5′ mRNA sequence”, XP002442734.
Database EMBL, Jun. 2, 2004, EBI Accession No. EMBL:CN811083, Fg03—12c23—R, fg03)AAFC—ECORC—Fusarium—graminearum—mycelium—trichothecene—prodcutr ionGibberella zeaecDNA clone Fg03—12c23, mRNA sequence, XP002442735.

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