Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2000-07-21
2004-05-18
Ketter, James (Department: 1636)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S320100, C435S455000, C435S366000
Reexamination Certificate
active
06737523
ABSTRACT:
BACKGROUND OF THE INVENTION
Throughout this application, various publications are referenced by author and date within the text. Full citations for these publications may be found listed alphabetically at the end of the specification immediately preceding the claims. All patents, patent applications and publications cited herein, whether supra or infra, are hereby incorporated by reference in their entirety. The disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art as known to those skilled therein as of the date of the invention described and claimed herein.
SUMMARY OF THE INVENTION
This invention provides for an isolated nucleic acid comprising a PEG-3 promoter comprising the nucleotide sequence beginning with the guanosine (G) at position −270 and ending with the cytosine (C) at position +194 of
FIG. 2
(nucleotides 1507-1970 of SEQ ID NO:1). The invention also provides for a method for identifying an agent which modulates PEG-3 promoter activity in a cell which comprises: (a) contacting the cell with the agent wherein the cell comprises a nucleic acid comprising a PEG-3 promoter operatively linked to a reporter gene; (b) measuring the level of reporter gene expression in the cell; and (c) comparing the expression level measured in step (b) with the reporter gene expression level measured in an identical cell in the absence of the agent, wherein a lower expression level measured in the presence of the agent is indicative of an agent that inhibits PEG-3 promoter activity and wherein a higher expression level measured in the presence of the agent is indicative of an agent that enhances PEG-3 promoter activity, thereby identifying an agent which modulates PEG-3 promoter activity in the cell. The invention provides a method for treating cancer in a subject which comprises administering a nucleic acid comprising a PEG-3 promoter operatively linked to a gene-of-interest wherein the gene of interest is selectively expressed in cancerous cells in the subject and such expression results in growth suppression or death of the cancerous cells, thereby treating cancer in the subject.
REFERENCES:
patent: WO 98/42315 (1998-10-01), None
Hollander et al (1997) Journal of Biological Chemistry 272:13731-13737.*
Hollander et al. Database GenEMBL. Accession No. U83984, Jul. 7, 1998. Accessed Apr. 6, 2002.*
Fisher et al. Database GENESEQ. Accession No. AAV65766, Feb. 2, 1999. Accessed Apr. 6, 2002.*
Verma et al (1997) Nature 389:239-242.*
Palu et al (1999) J. Biotechnol. 68: 1-13.*
Luo et al (2000) Nature Biotechnology 18:33-37.*
Fox, ASM News, Feb. 2000, 66 (2): 1-3.*
G.J., Young, C.S.H. and Fisher, P.B., PEG-3,A Nontransforming Cancer Progression Gene, Is A positive Regulator Of Cancer Aggressiveness and Angiogenesis, (1999),Proc. Natl. Acad. Sci. USA, 96(26):15115-20 (Exhibit B).
Gopalkrishnan, R.V., Christiansen, K., Goldstein, N.I., DePinho, R.A. and Fisher, P.B.,Use Of The Human EF-1 Alpha Promoter For Expression Can Significantly Increase Success In Establishing Stable Cell Lines With Consistent Expression: A Study Using The Tetracycline-inducible System In Human Cancer Cells(1999)Nucl. Acids Res., 27(24):4775-82 (Exhibit C).
Jiang, H. and Fisher, P.B., (1993)Use Of A Sensitive And Efficient Subtraction Hybridization Protocol For The Identification Of Genes Differentially Regulated During The Induction Of Differentiation In Human Melanoma Cells, Mol. Cell. Different., 1(3):285-299 (Exhibit D).
Kang, D.-c., Motwani, M. and Fisher, P.B.,Role Of The Transcriptional Factor AP-1 In Melanoma Differentiation(Review), (1998)Intl. J. Oncology, 13:1117-1126 (Exhibit E).
Kang, D.-c., LaFrance, R., Su, Z.-z. and Fisher, P.B.,Reciprocal Subtraction Differential RNA Display: An Efficient And Rapid Procedure For Isolating Differentially Expressed Gene Sequences, (1998) Proc.Natl. Acad. Sci. USA, 95(23):13788 (Exhibit F).
Seth. A., Ascione, R., Fisher, R. J., Mavrothalassitis, G.J., Bhat, N.K. and Papas, T.S.,The ets Gene Family(1992)Cell Growth&Different., 3(5):327-334 (Exhibit G).
Su, Z.-z., Yemul, S., Estabrook, A., Zimmer, S.G., Friedman, R.M. and Fisher, P.B.,Transcriptional Switching Model For The Regulation Of Tumorigenesis And Metastasis by The Has-ras Oncogene: Transcriptional Changes In The Has-ras Tumor Suppressor Gene Lysyl Oxidase, (1995)Intl. J. Oncology, 7:1279 (Exhibit H).
Su. Z.-z., Shen, R., O'Brian, C.A. and Fisher, P.B.,Induction Of Transformation Progression In Type 5 Adenovirus-transformed Rat Embryo Cells By A Cloned Protein Kinase C Beta 1 Gene And Reversal Of Progression By 5-Azacytidine, (1994)Oncogene, 9(4):1123-32 (Exhibit I).
Su, Z.-z., Shi, Y. and Fisher, P.B.,Subtraction Hybridization Identifies a Transformation Progression-Associated Gene PEG-3 With Sequence Homology To a Growth Arrest And DNA Damage-inducible Gene(1997)Proc. Natl. Acad. Sci. USA, 94:(17) 9125-30 (Exhibit J).
Fisher Paul B.
Su Zao-Zhong
Baker & Botts L.L.P.
Ketter James
Lambertson David
The Trustees of Columbia University in the City of New York
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