Nucleic acids comprising a post-transcriptional regulatory...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C424S185100, C424S188100, C424S199100, C424S207100, C424S208100, C536S023100, C536S024100

Reexamination Certificate

active

06919442

ABSTRACT:
The invention provides a novel post-transcriptional regulatory element that can function as an RNA nucleo-cytoplasmic transport element. The invention also provides for an attenuated HIV-1 hybrid virus for use as a vaccine and a kit incorporating the hybrid virus. The kit also includes instructional material teaching the use of the vaccine, where the instructional material indicates that the vaccine is used for the prophylaxis or amelioration of HIV-1 infection in a mammal; that the vaccine is to be administered to a mammal in a therapeutically effective amount sufficient to express a viral protein; where the vaccine will not cause clinically significant CD4+cell depletion; and, the expression of the viral protein elicits an immune response to the attenuated HIV-1 virus. The invention further provides for a method for screening for post-transcriptional RNA nucleo-cytoplasmic transport element (NCTE) binding proteins.

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Nkengasong et al. Dual infection with human immunodeficiency virus type 1 and type 2 : impact on HIV type 1 viral load and immune activation markers in HIV-seropositve female sex workers in Abidjan, Ivory Coast. AIDS Research and Human Retroviruses (2000).
Greenberg AE. Possible protective effect of HIV-2 against incident HIV-1 infection: review of available epidemiological and in vitro data. AIDS (2001) vol. 15, No. 17, pp. 2319-2321.
Macola et al. Aids vaccine : are we ready for human efficacy trials ? Journal of the American Medical association (1994) vol. 272, No. 6, pp. 488-489.
Feinberg et al. Aids Vaccine Models : challenging challenge viruses. Nature Medicine (2002) vol. 8, pp. 207-210.

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