Nucleic acids coding for vacuolar invertases, vegetal cells...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S320100, C435S419000, C536S023600, C800S285000, C800S286000

Reexamination Certificate

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10450606

ABSTRACT:
The present invention relates to a nucleic acid molecule encoding a vacuolar invertase and its use for modifying the plant saccharose metabolism, in particular in the vacuole of the cell of a plant storage organ. The present invention also relates to a fragment of the nucleic acid molecule, a vector containing the nucleic acid molecule or a fragment thereof, as well as a host cell, in particular a plant cell that contains the nucleic acid molecule or a fragment thereof. The present invention also comprises a transgenic plant, methods for producing a transgenic plant, as well as methods for modifying the saccharose metabolism of a plant, in particular of a plant part.

REFERENCES:
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Klan et al. Plant Physiol. vol. 112:1321-1330. 1996.
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Mazzolini et al. Assaying synthetic ribozymes in plants: high level expression of a functional hammerhead structure filas to inhibit target gene activity in transiently trsnsformed protoplasts. Plant Molecular Biology. vol. 20. 1992. p. 715-731.
NCBI Sequence Viewer; X81793—C.rubrum ClN2 mRNA for Intracellular Invertase. Sep. 2, 1997.
R.J. Milling, et al.; “Synthesis of a Vocuolar Acid Invertase in Washed Discs of Storage Root Tissue of Red Beet (Beta Vulgaris L.);” Journal of Experimental Botany, vol. 44, No. 268, pp. 1687-1694, Nov. 1993.
Guo-Qing Tang, et al.; “Antisense Repression of Vacuolar and Cell Wall Invertase in Transgenic Carrot Alters Early Plant Development and Sucrose Partitioning;” The Plant Cell, vol. 11, pp. 177-189, Feb. 1999.
Julie Scholes, et al.; “The Impact of Reduced Vacuolar invertase Activity on the Photosynthetic and Carbohydrate Metabolism of Tomato;” Planta vol. 200, pp. 265-272, 1996.
Akio Ohyama, et al.; “Suppression of Acid Invertase Activity by Antisense RNA Modifies the Sugar Composition of Tomato Fruit;” Plant Cell Physiol. 36(2): 369-376 (1995).
Ellen M. Klann, et al.; “Antisense Acid Invertase (TIV1) Gene Alters Soluble Sugar Composition and Size in Transgenic Tomato Fruit;” Plant Physiol. (1996) 112: 1321-1330.
Peters KF, et al.; “Isolation and Genetic Manipulation of Invertase Genes in Sugarcane;” CSIRO Division of Tropical Crops and Pastures, Brisbane. 1996, pp. 127-129.

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