Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2005-08-30
2005-08-30
Rao, Manjunath N. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S004000, C435S006120, C435S041000, C435S069100, C435S183000, C435S252100, C435S320100, C530S350000, C536S023200, C536S023700
Reexamination Certificate
active
06936448
ABSTRACT:
A rat ganglioside GM1-specific α1→2fucosyltransferase is disclosed. Nucleotide sequences of a rat ganglioside GM1-specific α1→2fucosyltransferase, amino acid sequences of its encoded protein (including peptide or polypeptide), and derivatives thereof are described. Also described are fragments (and derivatives and analogs thereof) which comprise a domain of rat ganglioside GM1-specific α1→2fucosyltransferase with catalytic activity. Methods of production of rat ganglioside GM1-specific α1→2fucosyltransferase and derivatives and analogs thereof (e.g. by recombinant means) are provided. Methods of inhibiting the function of rat ganglioside GM1-specific α1→2fucosyltransferase (e.g. by means of antisense RNA) are provided. Methods of commercial scale use of the rat ganglioside GM1-specific α1→2fucosyltransferase in the production of fucosyl-saccharide compositions are described. Applications of these compositions, e.g. as additives for human nutritive compositions or immunotherapeutics for cancer, are disclosed.
REFERENCES:
patent: 2002/0127655 (2002-09-01), Holmes et al.
Holmes et al. (JBC, 1983, vol. 258(6):3706-3713).
Baumann, et al., “Neutral Fucolipids and Fucogangliosides of Rat Hepatoma HTC and H35 Cells, Rat Liver, and Hepatocytes,”Cancer Research39: 2637-2643 (1979).
Holmes & Hakomori, “Isolation and Characterization of a New Fucoganglioside Accumulated in Precancerous Rat Liver and in Rat Hepatoma Induced by N-2-Acetylaminofluorene,”J. Biol. Chem.257:7698-7703 (1982).
Holmes & Hakomori, “Enzymatic Basis for Changes in Fucoganglioside during Chemical Carcinogenesis,”J. Biol. Chem.258: 3706-3713 (1983).
Scribner, et al., “Use of 2-Acetamidophenanthrene and 2-Acetamidofluorene in Investigations of Mechanisms of Hepatocarcinogenesis,”Environ. Health Perspect.49: 81-86 (1983).
Nilsson, et al., “Fucosyl-Gm1—A Ganglioside Associated With Small Cell Lung Carcinomas,”Glycoconjugate J.1: 43-49 (1984).
Fredman, et al., “Binding Specificity of Monoclonal Antibodies to Ganglioside, Fuc-Gm1,”Biochim. Biophys. Acta875: 316-323 (1986).
Holmes & Hakomori, “The Chemical Carcinogen-Induced Enzyme, GDP-Fucose: GM1α1→2 Fucosyltransferese in Rat Liver and Hepatoma: Modulation by and Association with Phospholipids,”J. Biochem.101: 1095-1105 (1987).
Hakomori, “Aberrant Glycosylation in Tumors and Tumor-Associated Carbohydrate Antigens,”Adv. Cancer Res.52: 257-331 (1989).
Kusunoki, et al., “Discrimination of Human Dorsal Root Ganglion Cells by Anti-fucosyl GM1 Antibody,”Brain Res.494: 391-395 (1989).
Holmes, “GDP-fucose: GM1α1→2fucosyltransferase is Activated in Parenchymal Cells of Rat Liver During Early Stages of N-2-acetylaminofluorene Induced Hepatocarcinogenesis,”Carcingenesis11: 89-94 (1990).
Larsen, et al., “Molecular Cloning, Sequence, and Expression of a Human GDP-L-fucose: β-D-galactoside 2-α-L-fucosyltransferase cDNA that can Form the H Blood Group Antigen,”Proc. Natl. Acad. Sci. USA87: 6674-6678 (1990).
Kusunoki, et al., “Developmental Changes of Fucosylated Glycoconjugates in Rabbit Dorsal Root Ganglia,”Neurosci. Res.15: 74-80 (1992).
Henion, et al., “Defining the Minimal Size of Catalytically Active Primate α1,3 Galactosyltransferase: Structure—Function Studies on the Recombinant Truncated Enzyme,”Glycobiology4: 193-201 (1994).
Piau, et al., “Evidence of Two Distinct α(1,2)-fucosyltransferase Genes Differentially Expressed Throughout the Rat Colon,”Biochem. J.300: 623-626 (1994).
Hitoshi, et al., “Molecular Cloning and Expression of Two Types of Rabbit β-Galactoside α1,2-Fucosyltransferase,”J. Biol. Chem.270: 8844-8850 (1995).
Holmes, et al., “Structure-Function Analysis of Human α1→3Fucosyltransferases,”J. Biol. Chem.270: 8145-8151 (1995).
Kelly, et al., “Sequence and Expression of a Candidate for the Human Secretor Blood Group α(1,2) Fucosyltransferase Gene (FUT2),”J. Biol. Chem.270: 4640-4649 (1995).
Hitoshi, et al., “Expression of the β-Galactoside α1,2-Fucosyltransferase Gene Suppresses Axonal Outgrowth of Neuro2a Neuroblastoma Cells,”J. Neurochem.66: 1633-1640 (1996).
Hitoshi, et al., “Molecular Cloning and Expression of a Third Type of Rabbit GDP-L-Fucose:β-D-Galactoside 2-α-L Fucosyltransferase,”J. Biol. Chem.271: 16975-16981 (1996).
Koda, et al., “Structure and Expression of H-type GDL-L-Fucose:β-D-Galactoside 2-α-L-Fucosyltransferase Gene (FUT1),”J. Biol. Chem.272: 7501-7505 (1997).
Koda, et al., “Structure and Expression of the Gene Encoding Secretor-type Galactoside 2-α-L-fucosyltransferase (FUT2),”Eur. J. Biochem.246: 750-755 (1997).
Sherwood & Holmes, “Cloning and Expression of the Catalytic Domain from Rat Hepatoma H35 Cell GDP-Fucose:GM1α1→2 Fucosyltransferase, an Enzyme Which is Activated during Early Stages of Chemical Carcinogenesis in Rat Liver,”Arch. Biochem. Biophys.355: 215-221 (1998).
Holmes Eric H.
Sherwood Anne L.
Northwest Biotherapeutics, Inc.
Rao Manjunath N.
Townsend and Townsend / and Crew LLP
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