Nucleic acid test article and its use to detect a predetermined

Details Nucleic acid test article and its use to detect a predetermined Nucleic acid test article and its use to detect a predetermined

1994-09-02

1996-05-07

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2432, C12Q 168, C12P 1934

Patent

active

055145507

ABSTRACT:
A nucleic acid test article can be used to detect a targeted nucleic acid found in a specimen. The test article includes a substrate having two opposing surfaces and a water-insoluble nucleic acid probe attached in a distinct zone of one of the surfaces. The probe is prepared from a water-insoluble particle to which is covalently attached an oligonucleotide which is complementary to the targeted nucleic acid. Substantially none of the probe is embedded within the surface of the substrate. Particularly useful test articles have a multiplicity of water-insoluble probes located in distinct zones on one of the substrate surfaces. These probes are useful for the detection of a multiplicity of targeted nucleic acids, particularly after amplification by polymerase chain reaction.

REFERENCES:
patent: 4358535 (1986-05-01), Falkow et al.
patent: 4563419 (1986-01-01), Ranki et al.
patent: 4673657 (1987-06-01), Christian
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Millis
patent: 4727019 (1988-02-01), Valkirs et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4988617 (1991-01-01), Landegren et al.
Matthews et al, Analytical Biochemistry, vol. 169, p. 1-25, Feb. 1988.

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