Nucleic acid sequencing with solid phase capturable terminators

Details Nucleic acid sequencing with solid phase capturable terminators Nucleic acid sequencing with solid phase capturable terminators

1997-01-15

1999-03-02

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 536 231, 536 253, C12Q 168, C12P 1934

Patent

active

058769366

ABSTRACT:
Methods of enzymatic nucleic acid sequencing are provided in which solid-phase capturable chain terminators are employed. In the subject methods, sequencing fragments are generated, where the fragments comprise capturable chain terminators. The fragments are then captured on a solid phase and separated from the remaining components of the sequencing reaction. The fragments are then released from the solid phase, size separated and detected to yield sequencing data from which the sequence of the nucleic acid is determined.

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