Chemistry: molecular biology and microbiology – Apparatus
Reexamination Certificate
2004-07-07
2008-12-16
Strzelecka, Teresa E (Department: 1637)
Chemistry: molecular biology and microbiology
Apparatus
C435S287200, C435S287900, C435S288700, C435S006120, C435S091200, C536S023100
Reexamination Certificate
active
07465578
ABSTRACT:
The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.
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Berlin Andrew
Kirch Steven J.
Neubauer Gabi
Rao Valluri
Yamakawa Mineo
Darby & Darby PC
Intel Corporation
Strzelecka Teresa E
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