Nucleic acid sequences specific for mycbacterium kansasii

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 2432, C12Q 168, C12P 1934, C07H 2100

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active

055188842

ABSTRACT:
Oligonucleotide probes and primers which exhibit M. kansasii-specificity in nucleic acid hybridization assays and in nucleic acid amplification reactions are provided. The full-length M. kansasii-specific sequence, identified herein as clone MK7, is 493 base pairs in length and has a GC content of 63%. Several M. kansasii-specific subsequences of MK7 are also provided. The probes and primers are useful in assays for species-specific detection and identification of M. kansasii.

REFERENCES:
Huang, et al 1991, J. Clin. Microbiol., 29, 2125-2129.
Ross, et al 1992, J.Clin. Microbiol., 30, 2930-2933.
Rogall et al. 1990, J. Gen. Microbiol., 136, 1915-1920.
Boddinghaus et al 1990, J. Clin. Microbiol., 28, 1751-1759.
Yang et al. 1993, J. Clin. Microbiol., 31, 2769-2772.
Gen Bank Loc; ORZCAMA, BMACTAZ, XLACASR, HUMNB1, SLMACT 35.

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