Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound
Reexamination Certificate
2007-07-10
2007-07-10
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing nitrogen-containing organic compound
C435S069100, C435S091100, C435S106000, C435S191000, C435S193000, C435S231000, C435S227000, C435S252300, C435S320100, C435S136000, C536S023200
Reexamination Certificate
active
10484681
ABSTRACT:
The invention relates to nucleic acid sequence encoding enantioselective amides with an amino acid sequence of SEQ ID NO: 2. The invention also relates to a process for fermentation, comprising a batch and a feed phase, of a microorganism in a fermentation medium, wherein the microorganism expresses a nucleic acid according to the invention and wherein between 0.5 and 50 mg of Zn2+/ml of fermentation medium is fed during the fermentation. The invention also relates to a process for the preparation of enatiomerically enriched carboxylic acid and/or an enatiomerically enriched carboxylic acid amide, in which a mixture of corresponding D- and L-carboxylic acid amides is contacted with a expression product according to the invention in the presence of 0.01 mM–100 mM Zn2+, whereby one of the enantiomers of the carboxylic acid amide is enatiomerically hydrolysed to from corresponding enatiomerically enriched carboxylic acid, while the other enantiomer of the carboxylic acid amide remains unchanged.
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Korevaar Cornelis Gerrit Nijs
Sonke Theodorus
Tandler Renate Francisca
Van Assema Friso Bernard Jan
Van Der Pol Rudolf
DSM IP Assets B.V.
Nixon & Vanderhye P.C.
Prouty Rebecca E.
Raghu Ganapathirama
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