Nucleic acid sequences encoding dendroides antifreeze proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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536 235, 536 2431, C12P 2102, C07H 2104

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056270518

ABSTRACT:
The present invention is directed to nucleic acid sequences encoding Dendroides canadensis thermal hysteresis proteins. The THPs of Dendroides have significantly greater thermal hysteresis activity than any other known anti-freeze protein. The thermal hysteresis activity of the purified THPs can be further enhanced by combining the THPs with various "activating" compounds.

REFERENCES:
J.G. Duman et al., "Hemolymph proteins involved in insect subzero tolerance: Ice nucleators and antifreeze proteins", In Insects at Low Temperatures, Chapman and Hall (N.Y.), pp. 94-127 (1991).
D.W. Wu et al., "Enhancement of insect antifreeze protein activity by antibodies", Biochem. Biophys. Acta 1076, 416-420 (1991).
J.G. Duman et al., "Adaptations of insects to subzero temperatures", Quart. Rev. Biol. 66, (4) pp. 387-410 (1991).
J.G. Duman et al., "Hemolymph Proteins involved in the cold tolerance of terrestrial arthropods: Antifreeze and Ice Nucleator Proteins". In Water and Life, Springer-Verlag (Berlin), Chapters 17 & 18 pp. 282-315, (1992).
J.G. Duman et al., "Thermal hysteresis proteins". Advances in Low Temperature Biology, vol. 2, pp. 131-182, (1993).
D. Tursman et al., "Cryoprotective effects of Thermal Hysteresis Protein on Survivorship of Frozen Gut Cells from the Freeze Tolerant Centipede Lithobius forficatus", Journal of Experimental Zoology, vol. 272, pp. 249-257 (1995).
D.W. Wu et al., "Activation of antifreeze proteins from larvae of the beetle Dendroides canadensis", Comp. Physiol. B, vol. 161, pp. 279-283 (1991).
D.W. Wu et al., "Purification and characterization of antifreeze proteins from larvae of the beetle Dendroides canadensis", Comp. Physiol. B, vol. 161, pp. 271-278 (1991).

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