Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Patent
1994-06-10
1997-10-28
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
536 232, C12N 988, C12N 1560
Patent
active
056817339
ABSTRACT:
The present invention describes the isolation and sequence of genes from Flavobacterium heparinum encoding heparin and heparan sulfate degrading enzymes, heparinase II and heparinase III (EC 4.2.2.8). It further describes a method of expressing and an expression for heparinases I, II and III using a modified ribosome binding region derived from a promoter from glycosaminoglycan lyase genes of F. heparinum. Also, a multi-step protein purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography and hydroxylapatite chromatography is outlined. Antibodies against a post-translational modification moiety common to Flavobacterium heparinum proteins and a method to obtain antibodies specific to these moieties and to the amino acid sequences of heparinases I, II and III are described.
REFERENCES:
patent: 5389539 (1995-02-01), Sasisekharan et al.
Lohse, et al., The Journal of Biological Chemistry, vol. 267:34, pp. 24347-24335 (1992).
Sasisekharan, et al., Proc. Natl. Acad. Sci., USA, vol. 90, pp. 3660-3664 (1993).
Zimmermann, et al., Applied and Environmental Microbiology, vol. 56:11, pp. 2593-3594 (1990).
Bennett Clark
Blain Francoise
Gu Kangfu
Musil Roy
Su Hongsheng
Ibex Technologies
Prouty Rebecca E.
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