Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1997-07-25
1999-07-06
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
536 232, 536 241, 4353201, 43525233, 435232, C12N 1560, C12N 121, C12N 1563, C12N 988
Patent
active
059196939
ABSTRACT:
The present invention describes the isolation and sequence of genes from Flavobacterium heparinum encoding heparin and heparan sulfate degrading enzymes, heparinase II and heparinase III (EC 4.2.2.8). It further describes a method of expressing and an expression for heparinases I, II and III using a modified ribosome binding region derived from a promoter from glycosaminoglycan lyase genes of F. heparinum. Also, a multi-step protein purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography and hydroxylapatite chromatography is outlined. Antibodies against a post-translational modification moiety common to Flavobacterium heparinum proteins and a method to obtain antibodies specific to these moieties and to the amino acid sequences of heparinases I, II and III are described.
REFERENCES:
patent: 5169772 (1992-12-01), Zimmerman et al.
patent: 5262325 (1993-11-01), Zimmerman et al.
Choay et al. (1980) Thrombosis Res. 18:573-578.
Turnbull et al. (1992) J. Biol. Chem. 267:10337-10341.
Kjellen et al. (1991) Ann. Rev. Biochem. 60:443-475.
Burgess et al. (1989) Ann. Rev. Biochem. 58:575-606.
McClean (1916) Am. J. Physiol. 41:250-257.
Linhardt et al. (1986) Appl. Biochem. Biotechnol. 12:135-177.
Oldberg et al. (1980) Biochemistry 19:5755-5762.
Nakajima et al. (1984) J. Biol. Chem. 259:2283-2290.
Gaal et al. (1989) Biochem. Biophys. Res. Comm. 161:604-614.
Nakajuma et al. (1988) J. Cell. Biochem. 36:157-167.
Yang et al. (1985) J. Biol. Chem. 260(3):1849-1857.
Lohse et al. (1992) J. Biol. Chem. 267:24347-24355.
Desai et al. (1993) Arch. Biochem. Biophys. 306(2):461-468.
Bashkin et al. (1992) J. Cell Physiol. 151:126-137.
Chappell et al. J. Biol. Chem. 268(19):14168-14175.
St. Groth et al. (1980) J. Immunol. Methods 35:1-21.
Lutz et al. (1988) Exp. Cell. Res. 175:109-124.
Sternberger et al. (1970) J. Histochem. Cytochem. 18:315-333.
Bayer et al. (1979) Meth. Enzym. 62:308-315.
Engvall et al. (1972) Immunol. 109:129-135.
Goding (1976) J. Immunol. Meth. 13:215-226.
Galliher et al. (1981) Appl. Environ. Microbiol. 41(2):360-365.
Laemmli (1970) Nature 227:680-685.
Brosius et al. (1984) Proc. Natl. Acad. Sci. USA 81:6929-6993.
Shine et al. (1974) Proc. Natl. Acad. Sci. USA 71:1342-1346.
Baker et al. (1984) Proc. Natl. Acad. Sci. 81:6779-6783.
Sanger et al. (1978) Proc. Natl. Sci. 74:5463-5467.
Southern (1975) J. Mol. Biol. 98:503-517.
Yanisch-Perron et al. (1985) Gene 33:103-119.
Vierra et al. (1991) Gene 100:189-194.
Chang et al. (1978) J. Bact. 134:1141-1156.
Lohse, et al., The Journal of Biological Chemistry, vol. 267:34, pp. 24347-24355 (1992).
Sasisekharan, et al., Proc. Natl. Acad. Sci., USA, vol. 90, pp. 3660-3664 (1993).
Zimmermann, et al., Applied and Environmental Microbiology, vol. 56:11, pp. 2593-3594 (1990).
Bennett Clark
Blain Francoise
Gu Kangfu
Musil Roy
Su Hongsheng
IBEX Technologies Corp.
Prouty Rebecca E.
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