Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-02-21
2006-02-21
Fredman, Jeffrey (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S320100, C435S252800, C435S174000, C435S183000, C382S129000, C382S133000, C382S153000, C382S173000, C382S286000, C382S291000, C702S019000, C702S022000, C536S022100
Reexamination Certificate
active
07001721
ABSTRACT:
Disclosed are methods for determining the presence or absence of a target nucleic acid (e.g. DNA) sequence in a sample nucleic acid, the method comprising: (a) exposing the sample to a detection agent comprising a colloid metal surface associated with a SER(R)S active species (SAS) such as an azo dye and with a target binding species (TBS) which may be PNA which is complementary to the target, and (b) observing the sample agent mixture using SER(R)S to detect any surface enhancement of the label wherein the binding of the TBS to the target sequence causes surface enhancement SAS. The detection agent may be exposed to the sample in step (a) as two or more separate components and will generally comprise a first agent and a second agent each having a different TBS, each TBS being capable of binding to the target sequence, and wherein the binding of the first and second TBS to the target sequence brings a metal surface associated with each TBS into proximity thereby causing surface enhancement of an SAS associated with one or both of the metal surfaces.
REFERENCES:
patent: 5266498 (1993-11-01), Tarcha
patent: 5306403 (1994-04-01), Vo-Dinh
patent: 5721102 (1998-02-01), Vo-Dinh
patent: 6127120 (2000-10-01), Graham
patent: 0 667 398 (1995-08-01), None
patent: 0 838 528 (1998-04-01), None
patent: WO 96/41181 (1996-12-01), None
patent: WO 97/05280 (1997-02-01), None
Kneipp, K. et al., “Surface Enhanced Raman Scattering (SERS) of nucleic Acids Adsorbed on Colloidal Silver Particles”; Journal of Molecular Structure, 145: 173-179 (1986) [Abstract].
Munro, C.H. et al., “Qualitative and Semi-quantitative Trace Analysis of Acidic Monoazo Dyes by Surface Enhanced Resonance Raman Scattering”; Analyst, 120: 993-1003 (1995).
Helmenstine, A. et al., “Measurement of DNA Adducts Using Surface-Enhanced Raman Spectroscopy”; Journal of Toxicology and Environmental Health, 40: 195-202 (1993).
Mirkin, C.A. et al., “A DNA-based method for rationally assembling nanoparticles Into macroscopic materials”; Nature, 382: 607-609 (1996).
Storhoff, J.J. et al., “One Pot Colormetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes”; J. Am. Chem. Soc., 120: 1959-1964 (1998) [Abstract].
Bethell, D. et al., “Nanotechnology and nucleotides”; Nature, 382: 581 (1996).
Alivisatos, A.P. et al., “Organization of ‘nanocrystal molecules’ using DNA”; Nature, 382: 609-611 (1996).
Munro, C.H. et al., “Characterization of the Surface of a Citrate-Reduced Colloid Optimized for Use as a Substrate for Surface-Enhanced Resonance Raman Scattering”; Langmuir, 11: 3712-3720 (1995).
Bertoluzza, A. et al., “Raman and Infrared Spectra of Spermidine and Spermine and their Hydrochlorides and Phosphates as a Basis for the Study of the Interactions Between Polyamines and Nucleic Acids”; Journal of Raman Spectroscopy, 14(6): 386-394 (1983).
Cotton, T.M., “Application of Surface-Enhanced Raman Spectroscopy to Biological Systems”; Journal of Raman Spectrocopy, 22: 729-742 (1991).
Egholm, M., “Spectrometry senses more than a small difference”; Nature Biotechnology, 15: 1346 (1997).
Graham, D. et al., “Selective Detection of Deoxyribonucleic Acid at Ultralow Concentrations by SERRS”; Analytical Chemistry, 69(22): 4703-4707 (1997).
Rodger, C. et al., “Surface-enhanced resonance-Raman scattering: an informative probe of surfaces”; J. Chem. Soc. Dalton Trans., 791-799 (1996).
Graham Duncan
Smith William Ewen
Whitcombe David Mark
Chakrabarti Arun
Dann, Dorfman, Herr & Skillman, P.C.
Fredman Jeffrey
University of Strathclyde
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