Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Reexamination Certificate
2005-03-22
2005-03-22
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
C435S320100, C536S024100, C536S023100, C424S093700
Reexamination Certificate
active
06869779
ABSTRACT:
Nucleic acid sequences for enhancing expression of a useful gene, which car increase the production of the gene product by enhancing gene expression, comprising a 5′-untranslated region of a viral gene or a fragment or a variant thereof, vectors comprising the nucleic acid sequence, host cells transformed or transfected with the vector, and methods for enhancing expression of a useful gene with the vector are provided. In addition, the sequences of the present invention can be utilized for screening an agent that interacts with IRES elements, and of an IRES-dependent translation initiator, as well as for treating diseases resulting from reduction of cap-dependent mRNA translation or reduction of IRES activity, and for determining severity of hepatitis C.
REFERENCES:
patent: 7-69899 (1995-03-01), None
patent: 10-327871 (1998-12-01), None
patent: WO 9811241 (1998-03-01), None
Verma et al., Gene therapy-promises, problems and prospects, 1997, Nature, vol. 389, pp. 239-242.*
Marshall, gene therapy's growing pains, 1995, Science, vol. 269, pp. 1050-1055.*
Orkin et al., Report and recommendations of the panel to assess the NIH investment in research gene therapy, 1995, NIH.*
Borman et al., Picornavirus internal ribosome entry segments: comparison of translation efficiency and the requirements for optimal internal initiation . . . , 1995, Nucleic Acids Reseach, vol. 23, pp. 3656-3663.*
Yoo et al., 5′ end-dependent translation initiation of hepatitis C viral RNA and the presence of putative positive and negative translational control elements within 5′ untranslated region, 1992, Virology, vol. 191, pp. 889-899.*
Collier et al., Translation efficiencies of the 5′ untranslated region from representatives of the six major genotypes of hepatitis C virus using a novel bicistronic reporter assay system, 1998, Journal of General Virology, vol. 79, pp. 2359-2366.*
Accession AB016785, Direct Submission, Virology 261 (2), 263-270 (1999).*
Brown et al., Secondary structure of the 5′ nontranslated regions of hapatitis C virus and positive genomic RNAs, 1992, Nucleic Acids Research, vol. 20, pp. 5041-5045.*
Dirks et al., Dicistronic transcription units for gene expression in mammalian cells, 1993, Gene, vol. 128, pp. 247-249.
Fukushi et al., The sequence element of the internal ribosome entry site and a 25-kilodalton cellular protein contribute to efficient internal initiation of translation of hepatitis C virus RNA, 1997, Journal of Virology, pp. 1662-1666.
Laporte et al., Comparative analysis of translation efficiencies of hepatitis C virus 5′ untranslated regions among intraindividual quasispecies present in chronic infection . . . , 2000, Journal of Virology, pp. 10827-10833.
Rijnbrand et al., The Influence of AUG Codons in the Hepatitis C Virus 5′ Nontranslated Region on Translation and Mapping of the Translation Initiation Window,Virology,226:47-56 (1996).
Genbank Accession No. AF054249, Yanagi et al., 1998.
Genbank Accession No. D00832.
Vennema et al., “Enhancement of the Vaccina Virus/Phage T7 RNA Polymerase Expression System Using Encephalomyocarditis Virus 5′-Untranslated Region Sequences,”Gene108:201-209 (1991).
Yanagi et al., “Transcripts of a Chimeric cDNA Clone of Hepatitis C Virus Genotype 1b Are Infectious in Vivo,”Virology244:161-172 (1998).
Ali et al., “The La Antigen Binds 5′ Noncoding Region of the Hepatitis C Virus RNA in the Context of the Initiator AUG Codon and Stimulates Internal Ribosome Entry Site-Mediated Translation”,Proc. Natl. Acad. Sci. USA,94(2249-2254) 1997.
Brown et al., “Secondary Structure of the 5′ Nontranslated Regions Of Hepatitis C Virus And Pestivirus Genomic RNAs”,Nucleic Acids Research,20:19(5041-5045) 1992.
Bukh et al., “Sequence Analysis of the 5′ Noncoding Region of Hepatitis C Virus”,Proc. Natl. Acad. Sci. USA,89:11(4942-4946)1992.
Buratti et al., “Functional Analysis of the Interaction Between HCV 5′UTR and Putative Subunits of Eukaryotic Translation Initiation Factor elF3,”Nucleic Acids Research,26:113(3179-3187)1998.
Dirks et al., “Dicistronic Transcription Units For Gene Expression In Mammalian Cells”,Gene,128:2(247-249)1993.
Fukishi et al., “The Sequence Element of the Internal Ribosome Entry Site and a 25-Kilodalton Cellular Protein Contribute to Efficient Internal Initiation of Translation of Hepatits C Virus RNA”,Journal of Virology,71:2(1662-1666)1997.
Gaines et al., “pIRES-CD4t, A Discistronic Expression Vector For MACS-or FACS-based Selection Of Transfected Cells”,Biotechniques,26:4(683-688)1999.
Hahm et al., “Hetergenous Nuclear Ribonucleoprotein L Interacts with the 3′ Border of the Internal Ribosomal Entry Site of Hepatitis C Virus”,Journal of Virology,72:11(8782-8788)1998.
Hijikata et al., “Gene Mapping of the Putative Structural Region of the Hepatitis C Virus Genome by in vitro Processing Analysis”,Proc. Natl. Acad. Sci. USA,88(5547-5551)1991.
Honda et al., “A Phylogenetically Conserved Stem-Loop Structure at the 5′ Border of the Internal Ribosome Entry Site of Hepatitis C Virus Is Required for Cap-Independent Viral Translation”,Journal of Virology,73:2(1165-1174)1999.
Ito et al., “The 3′-Untranslated Region of Hepatitis C Virus RNA Enhances Translation for an Internal Ribosomal Entry Site”,Journal of Virology,72:11(8789-8796)1998.
Jackson et al., “Internal Initiation of Translation of Picornavirus RNAs”,Molecular Biology Reports,19(147-159)1994.
Jackson et al., “Internal Initiation of Translation in Eukaryotes: The Picornavirus Paradigm and Beyond”,RNA,1(985-1000).
Kozak, M. “An Analysis of 5′-Noncoding Sequences from 699 Vertebrate Messenger RNAs”,Nucleic Acids Research,15:20(8125-8148)1987.
Martinez-Salas et al., “Functional Interactions In Internal Initiation Directed By Viral and Cellular IRES Elements”,Journal of General Virology,82(973-984)2001.
Paulin et al., “A Single Nucleotide Change in the c-mycInternal Ribosme Entry Segment Leads to Enhanced Binding of a Group of Protein Factors”,Nucleic Acids Research,26:13(3097-3103)1998.
Pestova et al., “A Prokaryotic-like Mode of Cytoplasmic Eukaryotic Ribosome Binding To the Initiation Codon During Internal Translation Initiation of Hepatitis C and Classical Swine Fever Virus RNAs”,Genes Dev.,12:1(67-83)1998.
Renyolds et al., “Unique Features of Internal Initiation of Hepatitis C Virus RNA Translation”,EMBO Journal,14:23(6010-6020)1995.
Sizova et al., “Specific Interaction of Eukaryotic Translation Initiation Factor 3 eith the 5′ Nontranslated Regions of Hepatitis C Virus and Classical Swine Fever Virus RNAs”,Journal of Virology,72:6(4775-4782).
Tsukiyama-Kohara et al., “Internal Ribosome Entry Site Within Hepatitis C Virus RNA”,Journal of Virology,66:3(1476-1483)1992.
International Search Report, International Application No.: PCT/JP99/03682, International Searching Authority/Japanese Patent Office (with Japanese language search report attached).
Yamada Osamu
Yoshida Hiroshi
Zhang Jing
FUSO Pharmaceutical Industries, Ltd.
Guzo David
Marshall & Gerstein & Borun LLP
Sullivan Daniel M.
LandOfFree
Nucleic acid sequence for potentiating the expression of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nucleic acid sequence for potentiating the expression of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid sequence for potentiating the expression of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3454303