Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-12-26
2006-12-26
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S023100, C536S024300
Reexamination Certificate
active
07153656
ABSTRACT:
Methods for rapidly detecting single or multiple sequence alleles in a sample nucleic acid are described. Provided are all of the oligonucleotide pairs capable of annealing specifically to a target allele and discriminating among possible sequences thereof, and ligating to each other to form an oligonucleotide complex when a particular sequence feature is present (or, alternatively, absent) in the sample nucleic acid. The design of each oligonucleotide pair permits the subsequent high-level PCR amplification of a specific amplicon when the oligonucleotide complex is formed, but not when the oligonucleotide complex is not formed. The presence or absence of the specific amplicon is used to detect the allele. Detection of the specific amplicon may be achieved using a variety of methods well known in the art, including without limitation, oligonucleotide capture onto DNA chips or microarrays, oligonucleotide capture onto beads or microspheres, electrophoresis, and mass spectrometry. Various labels and address-capture tags may be employed in the amplicon detection step of multiplexed assays, as further described herein.
REFERENCES:
patent: 4770992 (1988-09-01), VandenEngh et al.
patent: 4988617 (1991-01-01), Landegren et al.
patent: 5629158 (1997-05-01), Uhlen
patent: 5639611 (1997-06-01), Wallace et al.
patent: 5679524 (1997-10-01), Nikiforov et al.
patent: 5736330 (1998-04-01), Fulton
patent: 5876924 (1999-03-01), Zhang et al.
patent: 5912148 (1999-06-01), Eggerding
patent: 6027889 (2000-02-01), Barany et al.
patent: 6235889 (2001-05-01), Ulanovsky
patent: 6238868 (2001-05-01), Carrino et al.
patent: 6287766 (2001-09-01), Nolan et al.
patent: 6312892 (2001-11-01), Barany et al.
patent: 6361944 (2002-03-01), Mirkin et al.
PCT/US99/13928 (Weiner) filed Jun. 22, 1999.
EP1131113 (Schouten) filed May 9, 2001.
Pastinen et al., 1997, Genome Research 7: 606.
Tobe et al., 1996, Nucleic Acids Res. 24: 3728.
Favis et al, 2000, Nature Biotech. 18: 561.
Landegren et al., 1988, Science 241: 1077 and.
Ugozzoli et a., 1992, GATA 9(4): 107.
U.S. Appl. No. 09/877,819, filed Jun. 7, 2001.
Nolan John P.
White P. Scott
Los Alamos National Security LLC
Sharples Kenneth K.
Whisenant Ethan
Wilson Ray G.
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