Nucleic acid sequence detection employing amplification probes

Details Nucleic acid sequence detection employing amplification probes Nucleic acid sequence detection employing amplification probes

1994-12-27

1997-04-01

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 536 231, 536 243, 536 2432, 935 8, 935 76, 935 77, 436 2, 436 94, 436805, 436905, C12Q 168, C12P 1934, C07H 2104, C12N 1500

Patent

active

056164645

ABSTRACT:
Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently bind to is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Each of the nucleic acids is initially present as single stranded to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. Kits are provided with the appropriate reagents for carrying out the subject method.

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Barany, Genetic Disease Detection and DNA Amplification Using Cloned Thermostable Ligase (Jan. 1991) Proc. Natl. Acad. Sci., vol. 88:189-193.

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