Nucleic acid sequence analysis with nucleoside-5'-O-(1-thiotriph

Details Nucleic acid sequence analysis with nucleoside-5'-O-(1-thiotriph Nucleic acid sequence analysis with nucleoside-5'-O-(1-thiotriph

1990-09-28

1993-02-16

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435968, 436501, 436800, 935 78, 935 88, C12Q 168

Patent

active

051870851

ABSTRACT:
A chain-termination method of nucleic acid sequence determination is provided wherein nucleoside triphosphate precursors are replaced with their 1-thiotriphosphate analogs in the polymerization step. This substitution results in more uniform bands of electrophoretically separated DNA fragments which, in turn, results in more accurate base determination.

REFERENCES:
Tabor et al, proc. Natl. Acad. Sci., vol. 86, pp. 4076-4080 (1989).
Tabor et al, Proc. Natl. Acad. Sci., vol. 84, pp. 4767-4771 (1987).
Innis et al, Proc. Natl. Acad. Sci., vol. 85, pp. 9436-9440 (1988).
Connell et al, BioTechniques, vol. 5, pp. 342-348 (1987).
Smith et al, Meth. Enzymol., vol. 155, pp. 260-301 (1987).
Eckstein, Ann Rev. Biochem., vol. 54, pp. 367-402 (1985).
Eckstein et al, TIBS, vol. 14, pp. 97-100 (1989).
Ansorge et al, Nucleic Acids Research, vol. 15, pp. 4593-4602 (1987).
Prober et al, Science, vol. 238, pp. 336-341 (1987).
Kambara et al, Biotechnology, vol. 6, pp. 816-821 (1988).
Labeit et al, DNA, vol. 5, pp. 173-177 (1986).
Tabor et al, J. Biol. Chem., vol. 265, pp. 8322-8328 (1990).

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