Nucleic acid sequence amplification method, composition and kit

Details Nucleic acid sequence amplification method, composition and kit Nucleic acid sequence amplification method, composition and kit

1993-12-02

1996-09-10

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 9151, 435 9153, 536 2432, 536 2433, 935 17, 935 18, 935 8, 935 78, C12P 1934, C12Q 168, C07H 2104

Patent

active

055545165

ABSTRACT:
A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer. To effect the amplification, a supply of a single promoter-primer having a promoter and a primer complementary to the 3'-end of the target sequence, and a reverse transcriptase and an RNA polymerase are provided to a mixture including the target sequence; the amplification proceeds accordingly. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

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