Nucleic acid separation method

Details Nucleic acid separation method Nucleic acid separation method

1990-06-04

1992-03-17

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4351721, 4351723, 435259, 536 27, 935 19, 935 20, 935 21, C12P 1934, C12N 106

Patent

active

050968181

ABSTRACT:
An improved method for isolating and purifying nucleic acid from cell culture media contemplating adding to resuspended cell solutions a lysing solution and neutralizing/deproteinating agent without mechanical mixing, followed by centrifugation to partially pellet cellular debris, mixing the solution to complete the reaction, and additional centrifugation to fully pellet the debris.

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