Nucleic acid purification on silica gel and glass mixtures

Details Nucleic acid purification on silica gel and glass mixtures Nucleic acid purification on silica gel and glass mixtures

1995-06-07

1997-08-19

Kunz, Gary L.

Chemistry of inorganic compounds

Silicon or compound thereof

Oxygen containing

536 2542, 435 911, 428406, C03C 100, C03C 1200, C01B 3312, C01B 3314

Patent

active

056585489

ABSTRACT:
The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids occur in vivo or in vitro. The compositions of the invention are mixtures of the silica materials silica gel and glass particles, particularly glass microfibers; such mixtures combined with chaotropic salts, such as guanidinium chloride or guanidinium thiocyanate; and suspensions of such mixtures in aqueous solutions of chaotropic salts. In the methods of the invention, an aqueous solution comprising nucleic acid is mixed with an aqueous solution of chaotropic salts and the resulting solution is contacted with a mixture of the silica materials, whereupon the nucleic acid in the solution binds to the silica materials. The chaotropic salts and components, other than the nucleic acid adsorbed to the silica materials, from the aqueous solution treated by the method of the invention are washed from the silica materials. Finally, the nucleic acid can be obtained by elution from the silica materials. The methods provide nucleic acid in water or buffer, such as TE buffer, free of contamination by any salt or macromolecule that would interfere with further processing or analysis.

REFERENCES:
patent: 5075430 (1991-12-01), Little
patent: 5155018 (1992-10-01), Gillespie et al.
Boom, et al., "Rapid and Simple Method for Purification of Nucleic Acids", Journal of Clinical Microbiology, vol. 28, No. 3, pp. 495-503, 1990.
Marko, et al., "A Procedure for the Large-Scale Isolation of Highly Purified Plasmid DNA Using Alkaline Extraction and Binding to Glass Powder", Analytical Biochemistry, vol. 121, pp. 382-387, 1982.
Chen, et al., "Recovery of DNA Segments from Agarose Gels",Analytical Biochemistry, vol. 101, pp. 339-341, 1980.
Vogelstein, et al., "Preparative and analytical purification of DNA from agarose", Proc. Natl. Acad. Sci.USA , vol. 76, No. 2, pp. 615-619, 1979.

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