Nucleic acid purification method

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

07655793

ABSTRACT:
Disclosed is a process for separating and/or purifying a nucleic acid by elution of the nucleic acid from anion exchange resins under conditions of high salt concentration and the presence in the eluant of an additive comprising guanidine, or a guanidine-like derivative. The process allows high recovery of nucleic acids from anion exchange resins without impairing the nucleic acid stability as compared with conventional ion exchange chromatographic procedures.

REFERENCES:
patent: 5561064 (1996-10-01), Marquet et al.
patent: 5567807 (1996-10-01), Craig et al.
patent: 5660984 (1997-08-01), Davis et al.
patent: 5707812 (1998-01-01), Horn et al.
patent: 5801237 (1998-09-01), Johansson
patent: 5856192 (1999-01-01), Bloch
patent: 5866428 (1999-02-01), Kim et al.
patent: 5990301 (1999-11-01), Colpan et al.
patent: 6020186 (2000-02-01), Henco et al.
patent: 6090288 (2000-07-01), Berglund et al.
patent: 6310199 (2001-10-01), Smith et al.
patent: 6410274 (2002-06-01), Bhikhabhai
patent: 197 46 874 (1999-04-01), None
patent: 0616639 (1998-11-01), None
patent: 1 243 649 (2002-09-01), None
patent: 0992583 (2003-03-01), None
patent: 1125943 (2004-10-01), None
patent: WO 97/29825 (1997-08-01), None
patent: WO 00/69872 (2000-11-01), None
patent: WO01/27331 (2001-04-01), None
patent: WO03/080834 (2003-10-01), None
patent: WO 2004/101809 (2004-11-01), None
Chiang, Chen-Li, et al., “Application of superparamagnetic nanoparticles in purification of plasmid DNA from bacterial cells”. Journal of Chromatography B (2005) 822, p. 54-60.
Bosch, F. X. (1985). “Studies on the development of the charge heterogeneity of the influenza virus glycoproteins”. Archives of Virology, 83, 311-317.
Endres, H. N., Johnson, J. A. C., Ross, C. A., Welp, J. K., Etzel, M. R. (2003). “Evaluation of an ion-exchange membrane for the purification of plasmid DNA”. Biotechnology and Applied Biochemistry, 37, 259-266.
Ferreira, G. N. M., Cabral, J. M. S. & Prazeres, D. M. F. (2000). “Anion exchange purification of plasmid DNA using expanded bed adsorption”. Bioseparation, 9(1), 1-6.
Ferreira, G. N. M., Cabral, J. M. S., & Prazeres, D. M. F., (2000). “Studies on the Batch Adsorption of Plasmid DNA onto Anion-Exchange Chromatographic Supports”. Biotechnology Progress, 16(3), 416-424.
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). “Molecular Cloning: A Laboratory Manual”. Cold Spring Harbor; New York: Cold Spring Harbor Laboratory Press.
Tseng, W. C., Ho, F. L. (2003). “Enhanced purification of plasmid DNA using Q-Sepharose by modulation of alcohol concentrations”. Journal of Chromatography B, 791(1-2), 263-272.
Urthaler, J., Buchinger, W. & Necina, R. (2005). “Improved downstream process for the production of plasmid DNA for gene therapy”. Acta Biochimica Polonica, 52(3), 703-711.
Delius, H., et al., “Separation of complementary strands of plasmid DNA using the biotin-avidin system and its application to the heteroduplex formation and RNA/DNA hybridizations in electron microscopy”. Nucleic Acids Research (1985), 13(15) 5457-5469.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Nucleic acid purification method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid purification method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid purification method will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4212588

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.