Nucleic acid purification

Details Nucleic acid purification Nucleic acid purification

2002-03-27

2010-10-12

Sisson, Bradley L (Department: 1634)

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

C435S006120, C436S094000, C536S023100

Reexamination Certificate

active

07811807

ABSTRACT:
A process for purifying plasmid DNA from a nucleic acid containing sample comprising plasmid DNA and contaminants, which process comprises a step of contaminant removal, comprising:(a) treating the sample to form a nucleic acid solution having a concentration of monovalent cations;(b) contacting the nucleic acid solution with an ultrafiltration membrane having a molecular weight exclusion limit of at least 30 kDa under conditions in which substantially no gel-layer forms and in which the concentration of monovalent cations is sufficiently high for a time sufficient to remove substantially all RNA and form a retentate containing plasmid DNA; and(c) collecting the retentate.

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