Nucleic acid probes that reveal hypervariable restriction fragme

Details Nucleic acid probes that reveal hypervariable restriction fragme Nucleic acid probes that reveal hypervariable restriction fragme

1990-08-30

1993-12-28

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4353201, 436501, 536 221, 536 231, 536 241, 536 242, 536 2431, 536 2432, 536 2433, 935 17, 935 78, C12Q 168

Patent

active

052738787

ABSTRACT:
The present invention relates to nucleic aid molecules which comprise subfragments of ABR gene sequence. In particular embodiments, the nucleic acid molecules of the invention comprise portions of nucleic acid sequence contained in plasmids pVNTR-A or pVNTR-B. The invention is based, in part, on the discovery that a Taq-1 fragment of pVNTR-B, an EcoRI/HindIII fragment of pVNTR-A and, in preferred embodiments, a combination of these two fragments may be used to demonstrate restriction fragment length polymorphisms in the DNA of human subjects. Such restriction fragment length polymorphisms may provide a "genetic fingerprint" which may be used to identify individual persons or to provide evidence of a filial relationship in paternity cases. The nucleic acid sequences of the invention offer the advantage of producing an easily readable pattern in restriction fragment polymorphism analysis.

REFERENCES:
Jeffreys et al. (1985) Nature, vol. 314, pp. 67-73.
Heisterkamp et al. (1989) Nucleic Acids Research, vol. 17, No. 21, pp. 8821-8831.
Sigma Chemical Co. Catalog, (1986), Sigma Chemical Co., St. Louis, Mo., p. 902.
Nakamura et al., 1987, Science, 235:1616-1622.
Jeffreys, 1986, "Hypervariable DNA and Genetic Fingerprints," Current Communications in Molecular Biology, pp. 57-61.
Frank, 1986, J. Theor. Biol. 122:303-309.
Wong et al., 1986, Nucleic Acids Research 14:4605-4616.

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