Nucleic acid probes of human papilloma virus

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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435 6, 435 91, 435948, 436501, 436811, 935 9, 935 16, 935 17, 935 78, 935 88, C12Q 168, C07H 2104, G01N 33571, C12P 1934

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049837281

ABSTRACT:
The present invention relates to probes of nucleic acids useful for detecting indifferently the various types of human papilloma virus, particularly HPV1a, HPV5, HPV6b, HPV8, HPV11, HPV16, HPV18 and HPV33, especially a probe comprising a labelled sequence of nucleic acids, characterized in that it comprises the oligomer of twelve nucleotides X-A-A-A-A-C-G-A-A-A-G-X, with X=T or U, or its complement by interchanging A and X on the one hand, C and G on the other hand. The present invention also relates to specific probes of nucleic acids for the detection of human papilloma for each of the types HPV1a, HPV5, HPV8, HPV11, HPV16, HPV18 and HPV33, as well as specific probes of sub-groups of the virus HPV16, HPV18, HPV33 or HPV16 and HPV18 only or again HPV5 and HPV8 only.

REFERENCES:
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Schwarz et al. (1983), EMBO Journal vol. 2, No. 12, pp. 2341-2348.
Saiki et al. (1985), Science, vol. 230, pp. 1350-1354.
Chemical Abstracts, vol. 107, 1987, p. 409, #232380e, Columbus, Ohio, U.S., S. Syrjanen et al.: "Comparison of In Situ DNA Hybridization Protocols Using Sulfur-35-Labeled and Biotin-Labeled Probes in Detection of Human Papillomavirus DNA Sequences" & Cancel Cells 1987, 5 (Papillomaviruses), 329-36.
Chemical Abstracts, vol. 104, 4/86, p. 340, resume #125877p, Columbus, Ohio, U.S., by J. K. McDougall et al.: "Detection of Viral DNA and RNA by In Situ Hybridization", and J. Histochem. Cytochem. 1986, 34(1), 33-8.
Chemical Abstracts, vol. 105, 1986, p. 527, No. 169878q, Columbus, Ohio, U.S., K. Milde et al.: "Detection of Papillomavirus DNA in Oral Papillomas and Carcinomas: Application of In Situ Hybridization with Biotinylated HPV 16 Probes" & J. Oral Pathol. 1986, 15(5), 292-6.

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