Nucleic acid probes immobilized on polystyrene surfaces

Details Nucleic acid probes immobilized on polystyrene surfaces Nucleic acid probes immobilized on polystyrene surfaces

1991-12-23

1998-05-05

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 243, 530350, 106124, C12Q 168, C07H 2104, C07K 1708, C08L 8900

Patent

active

057472440

ABSTRACT:
Nucleic acid probes are immobilized on polystyrene surfaces such as the wells of microtiter plates for use in solution phase nucleic acid sandwich hybridization assays, particularly those using large branched DNA amplification multimers, by: (a) cleansing the surface by washing it sequentially with a strong acid, a strong base, and water, (b) passively adsorbing a polypeptide having primary amino groups onto the cleansed surface, and (c) covalently bonding the probe to the adsorbed polypeptide via a base-stable bifunctional crosslinking agent, and (d) subjecting the surface to conditions that simulate the hybridization conditions used in the assay.

REFERENCES:
patent: 4657853 (1987-04-01), Freytag et al.
patent: 4657873 (1987-04-01), Gadow et al.
patent: 4868105 (1989-09-01), Urdea et al.
U.S. Patent Application Serial No. 07/558,897 (filed 27 Jul. 1990).
Maniatis et al., "Molecular Cloning a Laboratory Manual", Cold Spring Harbor Laboratory, New York, 1982, pp. 314-315.
Owen et al., J. Clin. Microbiol. 27 (10): 2338-2343 (Oct. 1989).

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