Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-01-19
1997-07-15
Arthur, Lisa B.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2433, 935 77, 935 78, C07H 2104
Patent
active
056484810
ABSTRACT:
The invention relates to methods of detection of bacteria of the genus Shigella and/or Enteroinvasive E. coli (EIEC) by use of a set of nucleic acid probes. The invention further relates to a set of Shigella specific chromosomal sequences and fragments and to probes derived from the Shigella specific fragments. Additionally, probes were derived from a sequence from the Shigella ompA gene. In particular, a series of probes, each approximately 40 nucleotides in length, were designed having specificity for Shigella or for Shigella and Enteroinvasive E. coli, and having utility in non-isotopic test formats which require amplification to achieve high sensitivity. Specific hybridization probe sets which are capable of detecting substantially all clinically significant serotypes of Shigella, as well as enteroinvasive strains of E. coli, are disclosed.
REFERENCES:
patent: 4816389 (1989-03-01), Sansonetti et al.
patent: 4992364 (1991-02-01), Sansonetti et al.
patent: 5084565 (1992-01-01), Parodos et al.
Venkatesan, M. et al., "Development and Testing of Invasion-Associated DNA Probes for Detection of Shigella spp. and Enteroinvasive Escherichia coli" J. Clin. Microbiol., 26(2): 261-266 (1988).
Venkatesan, M. et al., "Use of Shigella flexneri ipaC and ipaH Gene Sequences for the General Identification of Shigella spp. and Enteroinvasive Escherichia coli" J. Clin. Microbiol., 27(12): 2687-2691 (1989).
Taylor, D.N. et al., "Clinical and Microbiologic Features of Shigella and Enteroinvasive Escherichia coli Infections Detected by DNA Hybridization" J. Clin. Microbiol., 26(7): 1362-1366 (1988).
Frankel, G. et al., "Detection of Shigella in Feces Using DNA Amplification" J. Infect. Dis., 161(6): 1252-1256 (Jun. 1990).
Boileau, C.R. et al., "DNA Hybridization Technique to Detect Shigella Species and Enteroinvasive Escherichia coli " J. Clin. Microbiol., 20(5): 959-961 (1984).
Sethabutr, O. et al., "A Non-Radioactive DNA Probe to Identify Shigella and Enteroinvasive Escherichia coli in Stools of Children with Diarrhoea" Lancet., ii, pp. 1095-1097, Nov. 16, 1985.
Sethabutr, O. et al., "DNA Hybridization in the Identification of Enteroinvasive Escherichia coli and Shigella in . . . " (1985), In: Infection Diarrhoea in the Young, S. Tzipoti et al., eds., (Elsevier Science Publishers, B.V.), pp. 350-356.
Gomes, T.A.T. et al., "DNA Probes for Identification of Enteroinvasive Escherichia coli", J. Clin. Microbiol., 25(10): 2025-2027 (1987).
Small, P.L.C. and S. Falkow, "Development of a DNA Probe for the Virulence Plasmid of Shigella spp. and Enteroinvasive Escherichia coli." (1986) In: Microbiology-1986, Leive,L., ed. (Am. Soc. for Microbiol. Washington, D.C.) pp. 121-124.
Marich, J.E. et al., "The Detection of Shigella and Enteroinvasive E. coli (EIEC) Using Oligonucleotide DNA Probes" Abstr. Ann. Mtg. Am. Soc. Microbiol., 89: 112, abstract No. D-179 (1989).
Braun, G. and S.T. Cole, "The Nucleotide Sequence Coding for Major Outer Membrane Protein OmpA of Shigella dysenteriae", Nucl. Acids Res., 10(7): 2367-2378 (1982).
Buysse, J.M. et al., "Molecular Cloning of Invasion Plasmid Antigen (ipa) Genes from Shigella flexneri: Analysis of ipa Gene Products and Genetic Mapping", J. Bacteriol., 169(6): 2561-2569.
Morrissey, D.V. et al., "Nucleic Acid Hybridization Assays Employing dA-Tailed Capture Probes; I. Multiple Capture Methods," Analytical Biochemistry 181: 345-359 (1989).
Hunsaker, W.R. et al., "Nucleic Acid Hybridization Assays Employing dA-Tailed Capture Probes; II. Advanced Multiple Capture Methods," Analytical Biochemistry 181: 360-370 (1989).
Lizardi, P.M. et al., "Exponential Amplfication of Recombinant-RNA Hybridization Probes, " Bio/Technology 6: 1197-1202 (Oct. 1988).
Lomeli, H. et al., "Quantitative Assays Based on the Use of Replicatable Hybridization Probes," Clin. Chem. 35(9): 18261-1831 (1989).
Pritchard, C.G. and J.E. Stefano, "Amplified Detection of Viral Nucleic Acid at Subattomole Levels Using Q Beta Replicase," Ann. Biol. Clin. 48: 492-497 (1990).
Dale, D.C. and L.J. Mata, "Studies of Diarrheal Disease in Central America; XI. Intestinal Bacterial Flora in Malnourished Children with Shigellosis," Am. J. of Tropical Medicine and Hygiene 17(3): 397-403 (1968).
Thomson, S. "The Numbers of Pathogenic Bacilli in Faeces in Intestinal Diseases," Journal of Hygiene 53: 217-224 (1955).
Matsutani, Sachiko et al. (1987) Journal of Molecular Biology, vol. 196, pp. 445-455.
Welcher, A.A. et al. (1986) Nucleic Acids Research, vol. 14, No. 24, pp. 10027-10044.
Cleuziat, P. et al. (1990) FEMS Microbiology Letters. Vol. 72, pp.315-322.
Wood, P.K. Journal of Clinical Microbiology, vol. 24, pp. 498-500, (1986).
McCarty Janice
Parodos Kyriaki
Amoco Corporation
Arthur Lisa B.
Galloway Norval B.
LandOfFree
Nucleic acid probes for the detection of shigella does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nucleic acid probes for the detection of shigella, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid probes for the detection of shigella will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1493186