Nucleic acid probes for the detection of mycoplasma pneumoniae a

Details Nucleic acid probes for the detection of mycoplasma pneumoniae a Nucleic acid probes for the detection of mycoplasma pneumoniae a

1994-07-29

1996-09-03

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2432, C12Q 168, C07H 2100

Patent

active

055522793

ABSTRACT:
Nucleic acid probes are described for detecting the principle etiological agent of primary atypical pneumonia, Mycoplasma pneumoniae, or, optionally, Mycoplasma pneumoniae and Mycoplasma genitalium. Said probes are complementary to ribonucleic acid sequences found in these mycoplasmas and absent from other mycoplasma, other bacterial, animal, or plant genomes. As such, these probes can detect the rRNA, rDNA, or polymerase chain reaction amplification products from these mycoplasma species. This set of probes, plus the described amplification primers, circumscribe a method for detecting the etiological agents of atypical pneumonia, and for making a clinical diagnosis of this disease. This set of probes also circumscribes a method for identification of these infectious agents in culture media enrichments inoculated from clinical samples.

REFERENCES:
patent: 4581333 (1986-03-01), Kourilsky et al.
Dams et al., Nuc. Acid Res., 16:r87-r173 (1988).
Gobel et al., J. Gen. Microbiol., 133:1969-1974 (1987).
Hyman et al., J. Clin. Microbiol., 25:726-728 (1987).
Peterson et al., Nucleic Acid Res., 19:6029-6031 (1991).
Rogers et al., Proc. Natl. Acad. Sci. USA, 82:1160-1164 (1984).
Weisburg et al., J. Bacteriology, 171:6455 (1989).
Woese et al., Proc. Natl. Acad. Sci. USA, 77:494-498 (1980).
Genback Locus IDS: EOORGNB (1981), BACRGRRNB (1983), PARRN23S (1987), N80802 (1990), N80803 (1990).
EMBL Locus Record: MRCRRNAPN (1990).
GCG Word Search No. of Hits (Diagonals) on GENBANK LOCUS MYCRRNAPN.
GENBANK LOCI MYCRRNAPN, MYCRRNAPI, MYCRRNAN, MYCRRNAF, MYCRRNAI, MYCRRNAAG.
GENALIGN ALIGNMENT of MYCRRNAM, MYCRRNAPI, MYCRRNAN, MYCRRNAF, MYCRRNAI, MYCRRNAAG.
Gutell et al, A Complation of Large Subumit RNA Sequences Presented in a Structural Format Nucl. Acids Res. (1988) vol. 16 (Supplement) r175-r269.
GCG Search Printout for SEQ ID Numbers:1-20.

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