Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-04-26
1997-06-03
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 2432, C12Q 168
Patent
active
056353537
ABSTRACT:
Provided is an isolated double-stranded nucleic acid consisting essentially of the nucleotide sequences defined in the Sequence Listing by SEQ ID Nos:5-9. These are the ITS2 sequences for C. albicans, C. parapsilosis, C. tropicalis, C. glabrata and C. krusei. A method of diagnosing systemic candidiasis in a subject is also provided. The method comprises the steps of: (a) collecting blood from the subject into tubes containing detergent, polypropylene glycol, sodium poyantholesulfonate, and sodium ethylene diamine tetraacetic acid; (b) lysing Candida cells using ZYMOLYASE.RTM.-100T with agitation; (c) extracting and precipitating the DNA from the lysed cells; (d) amplifying the precipitated DNA using universal fungal primer pairs derived from the internal transcribed spacer regions of the Candida ribosomal DNA; and (e) detecting amplified DNA from Candida by hybridizing the amplified DNA with a probe that selectively hybridizes with Candida DNA, the presence of amplified DNA indicating systemic candidiasis.
REFERENCES:
Huffman et al. Authentication of ATCC Strains in the Saccharomyces-Cerevisiae Complex by PCR Fingerprinting, Exp Mycol 16(4):316-319, 1992.
Wickes et al J. of Gen. Microbiol. (1992) 138: 901-907.
Zakroff et al. Abstract No. 100930 Amer. Soc. for Microbiol. Gen. Meeting, Atlanta, GA May 1993.
Lott et al. Abstract Amer. Soc. for Microbiol. Gen. Meeting, Atlanta, GA May 1993.
Niesters et al. J. Clin. Microbiol. 31(4):904-910, Apr. 1993.
Hopfer et al. J. of Med. and Vet. 31:65-75, 1993.
Kan/Bennett Abstract 1627, Interscience Conf. on Anti-Microbial Agents Chemotherapy, Oct. 1992.
Rand/Houck Abstract 1628, Interscience Conf. on Anti-Microbial Agents Chemotherapy, Oct. 1992.
Crampin, A.C. Abstract, J. Med. Microbiol. 37 (supp.1) Abst. No. 283, Jul. 1992.
Lehmann et al. Abstract F-16, Gen. Meet. Am. Soc. Microiol. New Orleand, LA, May 1992.
Miyakawa et al. J. of Clin. Microbiol. 30(4):894-900, Apr. 1992.
Oren et al. Abstract of D-46 Gen. Meeting Am. soc. for Microbiol., Dallas, TX, May 1991.
Lott/Kuykendall Abstract F-78 Gen. Meeting Am. soc. for Microbiol., Dallas, TX, May 1991.
Barns et al. J. of Bacteriol. 173(7-8):2147-2731, 1991.
Jones, J.J. Clin. Microbiol. Rev. 3:32-45, 1990.
Buchman et al. Surgery 108:338-347, 1990.
White et al. PCR Protocols:A Guide to Methods and Applications Innis et al. Eds. Academic Press, Inc. pp. 315-322, 1990.
Hendricks et al. System. Appl. Microbiol. 12:223-229, 1989.
Yue et al. Abstract, Clinical Research 37(2):446A, 1989.
Lasker Brent
Lott Timothy J.
Morrison Christine J.
Reiss Errol
Zakroff Sandra
Horlick Kenneth R.
The United States of America as represented by the Department of
Tung Joyce
LandOfFree
Nucleic acid probes and methods for detecting Candida krusei cel does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nucleic acid probes and methods for detecting Candida krusei cel, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid probes and methods for detecting Candida krusei cel will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-390550