Nucleic acid probe detectable by specific nucleic acid binding p

Details Nucleic acid probe detectable by specific nucleic acid binding p Nucleic acid probe detectable by specific nucleic acid binding p

1984-10-19

1988-10-11

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 7, 435 34, 435 39, 436501, 436504, 436508, 436808, 436811, 935 76, 935 77, 935 78, C12Q 168

Patent

active

047771291

ABSTRACT:
A nucleic acid detection probe comprising a hybridizable single stranded portion of nucleic acid connected with a non-hybridizable, single or double stranded nucleic acid portion, the non-hybridizable portion preferably including a recognition site for binding by a particular protein. Such recognition site can be a region of singly or doubly stranded nucleic acid specific for a particular nucleic acid binding protein such as lac repressor protein or can be a modified nucleic acid region such as a unique antigenic determinant introduced by interaction of the region with a modifier compound such as an intercalating agent or a platinum-containing ligand. The probe-binding protein can be labeled for ease of detection and in the case of an antigenic determinant binding site can be labeled antibody.

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Accounts of Chemical Research, vol. 11, 1978, "Platinum Complexes: Probes of Polynucleotide Structure and Antitumor Drugs", Stephen J. Lippard, pp. 211-217.
"Antibodies elicited against cis-diamminedichloroplatinum (II)-modified DNA are specific for cis-diamminedichloroplatinum (II)-DNA adducts formed in vivo and in vitro", M. C. Poirier et al., Proc. Natl. Acad. Sci. U.S.A. 79 (1982), pp. 6443-6447.

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