Nucleic acid primers for amplification of a mycobacteria RNA tem

Details Nucleic acid primers for amplification of a mycobacteria RNA tem Nucleic acid primers for amplification of a mycobacteria RNA tem

1995-05-23

1999-07-20

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2432, 536 243, C12Q 168, C12P 1934, C07H 2104

Patent

active

059255188

ABSTRACT:
Disclosed is a pair of primers to amplify a homologous region of the 16S rRNA of Mycobacteria. The primer pair performs better than a number of other primer pairs in a transcription-based amplification system. The amplified RNA can be detected with known probes for Mycobacteria. The primers, methods and kits are especially useful as an aid in the diagnosis of M. tuberculosis.

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