Nucleic acid polymer/intercalator monolayer and methods of using

Details Nucleic acid polymer/intercalator monolayer and methods of using Nucleic acid polymer/intercalator monolayer and methods of using

1997-11-07

2000-02-01

Riley, Jezia

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

546102, 546104, 546105, 536 221, 536 231, 536 243, 436501, 436518, 436536, C12Q 168, C07D21900, C07H 2104, G01N 33566

Patent

active

060201312

ABSTRACT:
The present invention has an object to provide an easy method for detecting a nucleic acid polymer in aqueous phase.
The present invention provides a method for detecting the amount of nucleic acid polymer, which comprises the steps of modifying an intercalator to be amphiphilic by using a hydrophobic group, spreading the amphiphilic intercalator on an aqueous solution containing a nucleic acid polymer to form a monolayer of said nucleic acid polymer and said amphiphilic intercalator at the gas-water interface, and measuring surface pressures per unit area of said monolayer.

REFERENCES:
patent: 4563417 (1986-01-01), Albarella et al.
patent: 5194393 (1993-03-01), Hugl et al.
Masaru Tanaka et al., "Preparation of Nucleic DNA Monolayer Complex by Polyionic Complex Process", Hokkaido University regional winter meeting of the Japanese Chemical Society held Feb. 2, 1995. (with English Abstract).
Kajiyama et al., "Effect of Polycation in Subphase on Aggregation Structure of Monolayer and Langmuir-Blodgett Film of Anionic Amphiphile" Langmuir, vol. 9, pp. 760-765, 1993.
Uhlmann et al., "Antisense Oligonucleotides: A New Therapeutic Principle" Chemical Review, vol. 90, p. 543, 1990.
Yamagishi et al. Effects of an aliphatic tail on the interactions of an ionic dye and a polyelectrolyte. The system of N-alylated acridine orange and poly(styrenesulfonic acid). J. Phys. Chem., vol. 85, pp. 2129-2134, 1981.

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