Nucleic acid molecules which encode proteins having...

Details Nucleic acid molecules which encode proteins having... Nucleic acid molecules which encode proteins having...

2006-08-01

2006-08-01

Prouty, Rebecca (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

C435S097000, C536S023200

Reexamination Certificate

active

07083963

ABSTRACT:
Nucleic acid molecules are described encoding proteins with the enzymatic activity of a fructosyl transferase. These enzymes are fructosyl transferases (FFT). Moreover, vectors and host cells are described containing the nucleic acid molecules of the invention, in particular transformed plant cells, plant tissue and plants regenerable therefrom, which express the described FFT. Furthermore, methods for the production of long-chain inulin by using the described proteins, hosts, in particular the plant cells and/or FFT produced by them, are described.

REFERENCES:
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patent: WO 96/21023 (1996-07-01), None
Goblet, J.-P., et al. “Cichorium intybusfructan-fructan 1-fructosyltransferase (1-FFT) cDNA,” GenBank Accession No. U84398, Jan. 9, 1997.
Hellwege, E.M., et al., “Differences in Chain Length Distribution of Inulin fromCynara scolymusandHelianthus tuberosusare Reflected in a Transient Plant Expression System Using the Respective 1- FFT cDNAs,”FEBS Lett., 427, pp. 25-28 (1998).
Hellwege, E.M., et al., “Transgenic Potato Tubers Accumulate High Levels of 1-Kestose and Nystose: Functional Identification of a Sucrose 1-Fructosyltransferase of Artichoke (Cynara scolymus) Blossom Discs,”Plant J., 12(5), pp. 1057-1065 (1997).
Koops, A.J., et al., “Purification and Characterization of the Enzymes of Fructan Biosynthesis in Tubers ofHelianthus tuberosus‘Colombia’ 1. Fructan:Fructan Fructosyl Transferase,”J, Exp. Bot., 45, pp. 1623-1631 (1994).
Lüscher, M., et al., “Inulin Synthesis by a Combination of Purified Fructosyltransferases from Tubers ofHelianthus tuberosus,” FEBS Lett., 385, pp. 39-42 (1996).
van der Meer, I.M., et al., “Cloning of the Fructan Biosynthesis Pathway of Jerusalem Artichoke”,Plant J., 15(4), pp. 489-500 (1998).

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