Nucleic acid molecules, vectors, and host cells encoding...

Details Nucleic acid molecules, vectors, and host cells encoding... Nucleic acid molecules, vectors, and host cells encoding...

2011-07-19

2011-07-19

Fronda, Christian (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S320100, C530S350000, C536S023200

Reexamination Certificate

active

07981658

ABSTRACT:
The present invention provides a fluorescent protein having an amino acid sequence of the green fluorescent protein, the yellow fluorescent protein or mutants thereof wherein the 46thphenylalanine residue is substituted with a leucine residue. According to the present invention, there are provided novel GFP or YEP mutants having an excellent maturation efficacy and having a decreased sensitivity to both of H+and CI31.

REFERENCES:
patent: 4314936 (1982-02-01), Yaron et al.
patent: 4868103 (1989-09-01), Stavrianopoulos et al.
patent: 5070012 (1991-12-01), Nolan et al.
patent: 5134232 (1992-07-01), Tsien et al.
patent: 5264563 (1993-11-01), Huse
patent: 5283173 (1994-02-01), Fields et al.
patent: 5439797 (1995-08-01), Tsien et al.
patent: 5491084 (1996-02-01), Chalfie et al.
patent: 5599906 (1997-02-01), Dasmahapatra
patent: 5602021 (1997-02-01), Davis et al.
patent: 5605809 (1997-02-01), Komoriya et al.
patent: 5614191 (1997-03-01), Puri et al.
patent: 5624048 (1997-04-01), Sander et al.
patent: 5625048 (1997-04-01), Tsien et al.
patent: 5741668 (1998-04-01), Ward et al.
patent: 5777079 (1998-07-01), Tsien et al.
patent: 5795737 (1998-08-01), Seed et al.
patent: 5804387 (1998-09-01), Cormack et al.
patent: 5958713 (1999-09-01), Thastrup et al.
patent: 5968738 (1999-10-01), Anderson et al.
patent: 5981200 (1999-11-01), Tsien et al.
patent: 5994077 (1999-11-01), Valdivia et al.
patent: 5998204 (1999-12-01), Tsien et al.
patent: 6027881 (2000-02-01), Pavlakis et al.
patent: 6054321 (2000-04-01), Tsien et al.
patent: 6066476 (2000-05-01), Tsien et al.
patent: 6077707 (2000-06-01), Tsien et al.
patent: 6124128 (2000-09-01), Tsien et al.
patent: 6140132 (2000-10-01), Tsien et al.
patent: 6146826 (2000-11-01), Chalfie et al.
patent: 6172188 (2001-01-01), Thastrup et al.
patent: 6197928 (2001-03-01), Tsien et al.
patent: 6319669 (2001-11-01), Tsien et al.
patent: 6403374 (2002-06-01), Tsien et al.
patent: 6593135 (2003-07-01), Wachter et al.
patent: 6638732 (2003-10-01), Evans
patent: 6780975 (2004-08-01), Tsien et al.
patent: 6800733 (2004-10-01), Tsien et al.
patent: 6803188 (2004-10-01), Tsien et al.
patent: 7060869 (2006-06-01), Tsien et al.
patent: 7166424 (2007-01-01), Michnick et al.
patent: 2002/0164674 (2002-11-01), Tsien et al.
patent: 2005/0079525 (2005-04-01), Tsien et al.
patent: 2006/0112440 (2006-05-01), Tsien et al.
patent: 2007/0021598 (2007-01-01), Evans
patent: 0 428 000 (1991-05-01), None
patent: 1 104 769 (2001-06-01), None
patent: 1 238 982 (2002-09-01), None
patent: WO 91/01305 (1991-02-01), None
patent: WO 94/28166 (1994-12-01), None
patent: WO 94/28173 (1994-12-01), None
patent: WO 95/07463 (1995-03-01), None
patent: WO 95/21191 (1995-08-01), None
patent: WO 96/13607 (1996-05-01), None
patent: WO 96/23810 (1996-08-01), None
patent: WO 96/23898 (1996-08-01), None
patent: WO 96/27027 (1996-09-01), None
patent: WO 96/27675 (1996-09-01), None
patent: WO 97/11094 (1997-03-01), None
patent: WO 97/26333 (1997-07-01), None
patent: WO 97/28261 (1997-08-01), None
patent: WO 97/42320 (1997-11-01), None
patent: WO 98/02571 (1998-01-01), None
patent: WO 98/06737 (1998-02-01), None
patent: WO 98/14605 (1998-04-01), None
patent: WO 98/40477 (1998-09-01), None
patent: WO 98/47148 (1998-10-01), None
patent: WO 99/64592 (1999-12-01), None
patent: WO 01/90147 (2001-11-01), None
patent: WO 01/98338 (2001-12-01), None
patent: WO 02/085936 (2002-10-01), None
Chica et al. Curr Opin Biotechnol. Aug. 2005;16(4):378-84.
Sen et al. Appl Biochem Biotechnol. Dec. 2007;143(3):212-23.
Angleson et al.,Trends Neurosci., 20: 281-287 (1997).
Anderson et al.,Proc. Natl. Acad. Sci. U.S.A., 93(16): 8508-8511 (1996).
Baldwin et al.,Biochemistry, 29: 5509-5515 (1990).
Blondel,Protein Engineering, 4(4): 457-461 (1991).
Bouvier et al.,Methods in Enzymology, 248: 614-633 (1995).
Cadwell et al.,PCR Methods and Applications, 3: S136-S140 (1994).
Cartwright et al.,Yeast, 10: 497-508 (1994).
Cesare et al.,Ann. New York Acad. Sci., 976: 520-527 (2002).
Chalfie et al.,Science, 263: 802-805 (1994).
Chalfie,Photochemistry and Photobiology, 62(4): 651-656 (1995).
Chattoraj et al.,Proc. Natl. Acad. Sci. U.S.A., 93: 8362-8367 (1996).
Cheng et al.,Nature Biotechnology, 14: 606-609 (1996).
Cody et al.,Biochemistry, 32: 1212-1218 (1993).
Colbran et al.,J. Biol. Chem., 267: 9589-9594 (1992).
Crameri et al.,Nature Biotechnol., 14: 315-319 (1996).
Cubitt et al.,Trends in Biochem. Science, 20: 448-455 (1995).
Cubitt et al.,Methods in Cell Biology, 58: 19-30 (1999).
Delagrave et al.,Biotechnology, 11: 1548-1552 (1993).
Delagrave et al.,Biotechnology, 13: 151-154 (1995).
Deschamps et al.,Protein Expression and Purification, 6: 555-558 (1995).
Dickson et al.,Science: 274, 966-969 (1996).
Dopf et al.,Gene, 173: 39-44 (1996).
Dunn et al.,Methods in Enzymology, 241: 254-279 (1994).
Ehrig et al.,FEBS Letters, 367: 163-166 (1995).
Elsliger et al.,Biochemistry, 38: 5296-5301 (1999).
Geoghegan et al.,Bioconjugate Chem., 4: 537-544 (1993).
Giuliano et al.,Annual Review of Biophysics and Biomolecular Structure, 24: 405-434 (1995).
Graff et al.,The Journal of Biological Chemistry, 266(2): 14390-14398 (1991).
Griesbeck et al.,J. Biol. Chem., 276(31): 29188-29194 (2001).
Guo et al.,Proc. Natl. Acad. Sci. U.S.A., 101: 9205-9210 (2004).
Hardy et al., “Generic Variability and Alzheimer's Disease,”Amyloid Protein Precursor in Development, Aging and Alzheimer's Disease, 190-198 (1994).
Heikal et al.,Proc. Natl. Acad. Sci. U.S.A., 97(22): 11996-12001 (2000).
Heim et al.,Biochemistry, 91: 12501-12504 (1994).
Heim et al.,Nature, 373: 663-664 (1995).
Heim et al.,Current Biology, 6(2): 178-182 (1996).
Houdebine,J. Biotech., 34: 269-287 (1994).
Inouye et al.,FEBS Letters, 341: 277-280 (1994).
Jayaraman et al.,J. Biol. Chem., 275(9): 6047-6050 (2000).
Kain et al.,BioTechniques, 19(4): 650-655 (1995).
Kappel et al.,Current Opinion in Biotechnology, 3(5): 548-553 (1992).
Kemp et al.,TIBS, 15: 342-346 (1990)
Kilgard et al.,Nature, 373: 663-664 (1995).
Knight,Methods in Enzymology, 248: 18-34 (1995).
Kolberg,Journal of NIH Research, 5: 44-47 (1994).
Krafft et al.,Methods in Enzymology, 241: 70-86 (1994).
Kuner et al.,Neuron, 27: 447-459 (2000).
Lang et al.,Neuron, 18: 857-863 (1997).
Lee et al.,Proc. Natl. Acad. Sci. U.S.A., 91: 6413-6417 (1994).
Levine et al.,Comparative Biochemistry and Physiology, 72B(1): 77-85 (1982).
Marshall et al.,Neuron, 14: 211-215 (1995).
Matayoshi et al.,Science, 247: 954-958 (1990).
Misteli et al.,Nature Biotechnology, 15: 961-964 (1997).
Mitra et al.,Gene, 173: 13-17 (1996).
Miyawaki et al.,Nature, 388: 882-887 (1997).
Miyawaki et al.,Proc. Natl. Acad. Sci. U.S.A., 96: 2135-2140 (1999).
Miyawaki et al.,Methods in Enzymology, 327: 472-500 (2000).
Muhlrad et al.,Yeast, 8: 79-82 (1992).
Mullins et al.,J. Clin. Invest., 97(7): 1557-1560 (1996).
Mullins et al.,J. Clin. Invest., 98(11): S37-S40 (1996).
Murray,Theriogenol., 51(1): 149-159 (1999).
Nagai et al.,Proc. Natl. Acad. Sci. U.S.A., 98(6): 3197-3202 (2001).
Nagai et al.,Nature Biotechnology, 20: 87-90 (2002).
Niwa et al.,Proc. Natl. Acad. Sci. U.S.A., 93: 13617-13622 (1996).
Norris et al.,Plant Molecular Biology, 24: 673-677 (1994).
Ormo et al.,Science, 273: 1392-1395 (1996).
Palm et al.,Nature Struct. Biol., 4: 361-365 (1997).
Perozzo et al.,J. Biol. Chem., 263(16): 7713-7716 (1988).
Pouli et al.,Biochem J., 331: 669-675 (1998).
Prasher et al.,Gene, 111: 229-233 (1992).
Reid et al.,Biochemistry, 36: 6786-6791 (1997).
Sawano et al.,Nucl. Acids Res., 28(16): e78 (2000).
Seidah et al.,Methods in Enzymology, 244: 175-188 (1994).
Sigmund,Arterioscler. Thromb. Vasc. Biol., 20(6); 1425-1429 (2000).
Smith et al.,Methods in Enzymology, 244: 412-423 (1994).
Songyang et al.,Current Biology, 4(11): 973-982 (1994).
Stearns,Current Biology, 5: 262-264 (1995).
Steyer et al.,Nature Reviews Mol. Cell Biol., 2: 268-275 (2001).
Stokoe et al.,Biochemical Journal, 296: 843-849 (1993).
Stryer,Annual Review of Biochemistry, 47: 819-846 (1978).
Thornberry,Methods in Enzymology, 244: 615-631 (1994).
Tsien et al.,Trends in Cell Biology, 3: 242-245 (1993).
Tsien,Annu. Rev. Biochem.,

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