Nucleic acid molecules encoding Streptomyces 1AG3 serine...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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Reexamination Certificate

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08076468

ABSTRACT:
Certain aspects of this disclosure relate to an isolated protease, and cleaning compositions containing the same. In some embodiments, the protease may comprise an amino acid sequence that is at least 80% identical to the wild typeStreptomyces1AG3 protease. Isolated nucleic acid encoding the subject protease, recombinant nucleic acid containing the same and host cells containing the recombinant nucleic acid are also provided.

REFERENCES:
patent: 1 493 807 (2009-06-01), None
patent: WO98/55579 (1998-12-01), None
Rawlings and Barrett, Evolutionary families of metallopeptidases. Methods Enzymol. 1995;248:205-228.
Sidhu et al, J Biol Chem. Aug. 5, 1994;269(31):20167-71.Streptomyces griseusprotease C. A novel enzyme of the chymotrypsin superfamily.
Sigma, Inc, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, Product Information Sheet. May 29, 2003.
PIR—80 database Acc. No. A53669 from Sidhu et al, J Biol Chem. Aug. 5, 1994;269(31):20167-71. Alignment with SEQ ID No. 9.
Rawlings, N.D., et al., “Evolutionary families of metallopeptidases.”Methods Enzymol.248: 183-228 (1995).
Sidhu, S.S., et al., “Streptomyces griserusProtease C. A novel Enzyme of the Chymotrypsin Superfamily.” J. Biol. Chem. 269(31): 20167-20171 (1994).
Sigma, Inc., N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, Production Information Sheet, May 29, 2003.
PIR—80 Database Acc. No. A53669 from Sidhu et al.,J. Biol. Chem.269(31): 20167-20171, Alignment with SEQ ID No:9.
International Search Report and the Written Opinion of the International Searching Authority for International Application No. PCT/US08/81088 dated Feb. 9, 2009.

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