Nucleic acid molecules encoding red and green emitting...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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C435S252300, C435S252330, C435S320100, C536S023200

Reexamination Certificate

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10940177

ABSTRACT:
Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence ofPhrixotrhix vivianiiand red bioluminescence ofPhrixothrix hirtusare described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.

REFERENCES:
patent: 5700673 (1997-12-01), McElroy et al.
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Vadim R. Viviani, et al., “Bioluminescence and Biological Aspects of Brazilian Railroad-Worms (Coleoptera: Phengodidae),” Annals of the Entomological Society of America, vol. 90 No. 3, pp. 389-398 (1997).
VR Viviani, et al., “Cloning and Characterization of cDNA for the Larval Click-BeetlePyrearinus termitilluminans(Coleoptera:Elateridae) Luciferase,” Proceedings of the 10thInternational Symposium on Bioluminescence and Chemiluminescence, Bologna, Italy, John Wiley & Sons, New York, pp. 384-387 (1999).
Vadim R. Viviani, et al., “Cloning, Sequence Analysis, and Expression of ActivePhrixothrixRailroad-Worms Luciferases: Relationship between Bioluminescence Spectra and Primary Structures,” Biochemistry 1999, 38, pp. 8271-8279.
Roda, A., et al., “Bioluminescence and Chemiluminescence,” Proceedings of the 10thInternational Symposium on Bioluminescence and Chemiluminescence held at Bologna, Italy, Sep. 1998.
Viviani, V.R., et al., “Bioluminescence Color Determinants of Phrixothrix Railroad-worm Luciferases: Chimeric Luciferases, Site-directed Mutagenesis of Arg 215 and Guanidine effect,” Photochemistry and Photobiology, 72(2): 267-271 (2000).
Viviani, et al., “Cloning and Characterization of cDNAs for Phrixothrix spp (Coleoptera: Phenogodidae) Railroad-Worms Luciferases,” J. Biolumin Chemilumin., 12:239 (1998) Abstract from the 10thInternational Symposium on Bioluminescence & Clemiluminescence, Bologna, Italy Sep. 4-8, 1998.
Viviani, et al., “ThR26 is a Key Residue for Bioluminescence Spectra Determination in Beetle Luciferases,” Biochem. And Biophys. Research Commun., 280:1286-1291 (2001).
NCBI Accession, AF 139644 (Jun. 2, 1999).
NCBI Accession, AF 139645 (Jun. 2, 1999).

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