Nucleic acid molecules encoding red and green emitting...

Details Nucleic acid molecules encoding red and green emitting... Nucleic acid molecules encoding red and green emitting...

2005-11-08

2005-11-08

Slobodyansky, Elizabeth (Department: 1652)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S189000, C435S252300, C435S252330, C435S320100, C435S325000, C435S410000

Reexamination Certificate

active

06962986

ABSTRACT:
Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence ofPhrixotrhix vivianiiand red bioluminescence ofPhrixothrix hirtusare described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.

REFERENCES:
patent: 5700673 (1997-12-01), McElroy et al.
Viviani et al. (Jun. 29, 1999) Biochemistry, vol. 38 (26), pp. 8271-8279.
Viviani, V.R., et al., “Bioluminescence Color Determinants ofPhrixothrixRailroad-worm Luciferases: Chimeric Luciferases, Site-directed Mutagenesis of Arg 215 and Guanidine effect”,Photochemistry and Photobiology, 72(2):267-271 (2000).
GeneBank database (Jun. 2, 1999) Accession.
GeneBank database (Jun. 2, 1999) Accession.
Viviani et al., “Cloning and Characterization of cDNAs forPhrixothrixapp (Coleoptera:Phenogodidae) Railroad-Worms Luciferases”,J. Biolumin Chemilumin., 13:239 (1998) Abstract from the 10thInternational Symposium on Bioluminescence & Clemiluminescence, Bologna, Italy Sep. 4-8, 1998.
Viviani, et al., “Thr226 is a Key Residue for Bioluminescence Spectra Determination in Beetle Luciferases”,Biochem. and Biophys. Research Commun., 280:1286-1291 (2001).
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Vadim R. Viviani, et al., “Bioluminescence of Brazilian Fireflies (Coleoptera: Lampyridae): Spectral Distribution and pH Effects on Luciferase-Elicited Colors. Comparison with Elaterid and Phengodid Luciferases,” Photochemistry and Photobiology, vol. 62, No. 3, pp. 490-495 (1995).
Vadim R. Viviani, et al., “Bioluminescence and Biological Aspects of Brazilian Railroad-Worms (Coleoptera: Phengodidae),” Annals of the Entomological Society of America, vol. 90 No. 3, pp. 389-398 (1997).
VR Viviani et al., “Cloning and Characterization of cDNA for the Larval Click-BeetlePyrearinus termitilluminans(Coleoptera:Elateridae) Luciferase,” Proceedings of the 10thInternational Symposium on Bioluminescence and Chemiluminescence, Bologna, Italy, John Wiley & Sons, New York, pp. 384-387 (1999).

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