Nucleic acid molecules encoding novel parasitic helminth protein

Details Nucleic acid molecules encoding novel parasitic helminth protein Nucleic acid molecules encoding novel parasitic helminth protein

1993-08-19

1997-06-17

Sidberry, Hazel F.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 221, 536 231, 435 691, 435 693, 435 711, 4241841, 4241851, 4242651, 4242661, C07H 1900, C07H 2104, C12P 2104, A61K 3900

Patent

active

056398760

ABSTRACT:
The present invention relates to isolated parasitic helminth nucleic acid sequences capable of hybridizing, under stringent conditions, to at least a portion of D. immitis nucleic acid sequence p4 and/or to at least a portion of D. immitis nucleic acid sequence p22U; to isolated parasitic helminth proteins that are encoded by such parasitic helminth nucleic acid sequences and that are capable of selectively binding to at least one component of immune serum capable of inhibiting helminth development; and to antibodies raised against such isolated parasitic helminth proteins. The present invention also relates to therapeutic compositions comprising such isolated nucleic acid sequences, proteins and/or antibodies. The present invention also includes methods to produce and use such nucleic acids, proteins, antibodies and therapeutic compositions capable of protecting animals from parasitic helminth infection and, particularly, from heartworm infection.

REFERENCES:
patent: 4842999 (1989-06-01), Fuller et al.
Tripp et al Am J. Trop Med Hyg 49:217-18 1993 (Abstract only).
Grieve Immunoprophylotis pp. 187-190 Heartworm Symp. 1989.
Grieve et al, The Journ of Immun. 148:2511-2515 1992.
Young et al PNAS 80:1194-1196, 1983.
Culpepper et al, Mol. & Biochem. Porasitology 54:51-62 (1992).
Tannich et al, Mol. Biochem. Parasitol 55:225-228 1992 Database search attached.
Abraham, et al., "Passive Transfer of Protective Immunity to Larval Dirofilaria Immitis from Dogs to Balb/C Mice", pp. 254-257, 1991, J. Parasitol., Vol. 77(2).
Abraham, et al., "Genetic Control of Murine Immune Responses to Larval Dirofilaria Immitis", pp. 523-528, 1990, J. Parasitol., vol. 76(4).
Abraham, et al., "Dirofilaria Immitis: Molting Process of Third-Stage Larvae", pp. 314-322, 1990, Exp. Parasitol., vol. 70.
Abraham, et al., "Active and Passive Immunization of Mice Against Larval Dirofilaria Immitis", pp. 275-282, 1988, J. Parasitol., vol. 74(2).
Amiri, et al., "The Schistosomatium Douthitti Cerarial Elastase is Biochemically and Structurally Distinct from that of Schistosoma Mansoni, "pp. 113-120, 1988, Mol. Bio-chem. Parasitol, vol. 28.
Awobuluyi, et al. "Immunureactivity of Cloned Dirofilaria Immitis Proteins in Dogs Following Vaccination with Irradiated Infective Larvae," p. 139, 1989, 38th Annual Meeting, Am. J. Trop. Med. Hyg. Abstract #150, Dec.
Bianco, et al., "Developmentally Regulated Expression and Secretion of a Polymorphic Antigen by Onchocerca Infective-Stage Larvae", pp. 203-212, 1990, Mol. Biochem. Parasitol., vol. 39.
Blair, et al., "Immunization of Dogs Against Dirofilaria Immitis by Means of Chemically Abbreviated Infections", 1982, Fifth International Congress of Parasitol., Toronto, Canada, Aug.
Boyer, et al., "Differential Antigen Content and Isotype Recognition of O. Volvulvus Antigens from Nodules Removed from Guatemalan Children", p. 169, 1990, 39th Annual Meeting, Am. J. Trop. Med. Hyg., Abstract #221, Nov.
Chomczynski, et al., "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction", pp. 156-159, 1987, Anal. Biochem., vol. 162.
Coleman, et al., "Use of Implantable Intraperitoneal Diffusion Chambers to Study Bordetella Pertussis Pathogenesis: Growth and Toxin Production in Vivo", pp. 33-39, 1986, J. Infect. Dis., vol. 154(1), Jul.
Culpepper et al. "Molecular Characterization of a Dirofilaria Immitis cDNA Encoding a Highly Immunoreactive Antigen", pp. 51-62, 1992, Mol. Biochem. Parasitol., vol. 54.
Dalton, et al., "Thiol Proteases Released in Vitro by Fasciola Hepatica", pp. 161-166, 1989, Mol. Biochem. Parasitol., vol. 35.
Davis et al. "Purification and Biochemical and Immunologic Characterization of a 25KD Glycoprotein from the Surface of Dirofilaria Immitis Fourth Stage Larvae", p. 256, 1988, 37th Annual Meeting. Am. Soc. Trop. Med. Hyg., Abstract #404.
Delves, et al., "Neurosecretory-Like Material in 3rd-and 4th-Stage Dirofilaria Immitis Larvae (Nematoda: Filarioidea)", pp. 99-104, 1989 J. Parasitol., vol. 99.
Denham, "Vaccination Against Filarial Worms Using Radiation-Attenuated Vaccines", pp. 105-111, 1980, Inter. J. Nucl. Med. Biol., vol. 7.
Frank, et al., "Metabolic Labeling of Dirofilaria Immitis Third and Fourth-Stage Larvae and Their Excretory-Secretory Products", pp. 950-956, 1991, J. Parasitol., vol. 77(6).
Gamble, et al., "Purification of a 44 Kilodalton Protease which Mediates the Ecdysis of Infective Haemonchus Contortus Larvae", pp. 49-58 (1989), Mol. Biochem. Parasitol., vol. 33.
Grieve, et al., "Identification of Dirofilaria Immitis Larval Anitgens with Immunoprophylactic Potential Using Sera from Immune Dogs", pp. 2511-2515, 1992, J. Immunol., vol. 148(8), Apr.
Grieve, "Potential for Immunoprophylaxis Against Heartworm (Dirofilaria Immitis) Infection", pp. 187-190, 1989, Proc. Heartworm Symp.
Grieve, et al., "Induction of Protective Immunity in Dogs to Infection with Dirofilaria Immitis Using Chemically-Abbreviated Infections", pp. 373-379, 1988, Am. J. Trop. Med. Hyg., vol. 39(4).
Grieve, et al., "Epidemiology of Canine Heartworm Infection"pp. 220-246, 1983, Epidem. Rev., vol 5.
Hewick et al. "A Gas-Liquid Solid Phase Peptide and Protein Sequenator", pp. 7990-7997, 1981 J. Biol. Chem., vol. 256(15).
Hotez et al., "Isolation and Characterization of a Proteolytic Enzyme from the Adult Hookworm Ancylostoma Caninum", pp. 7343-7348, 1985, J. Biol. Chem., vol. 260(12).
Ibrahim, et al., "Antigen Shedding from the Surface of the Infective Stage Larvae of Dirofilaria Immitis", pp. 89-97, 1989, J. Parasitol., vol. 99.
Jwo, et al., "Fractionated Sera from Schistosoma Mansoni Infected Patients Confers Passive Protection in Mice", pp. 553-562, 1989, Am. J. Trop. Med. Hyg., vol. 41(5).
Kassis, et al., "Antibody-Dependent Complement-Mediated Killing of Schistosomula in Intraperitoneal Diffusion Chambers in Mice", pp. 1659-1662, 1979, J. Immunol., vol. 123(4), Oct.
Lackey, et al., "Extracellular Proteases Of Onchocerca", pp. 176-185, 1989, Exp. Parasitol., vol. 68.
Lal, et al, "Characterization of Stage-Specific Antigens of Infective of the Filarial Parasite Brugia Malayi", pp. 2032-2038, 1988, J. Immunol., vol. 140.
Maki, et at., "Demonstration of Carboxyl and Thiol Protease Activities in Adult Schistosoma Mansoni, Dirofilaria Immitis, Angiostrongylus Cantonensis and Ascaris Suum", pp. 31-37, 1986, J. Helminthol., vol. 60.
McKerrow, et al., "Proteinases From Invasive Larvae Of The Trematode Parasite Schistosoma Mansoni Degrade Connective-Tissue And Basement-Membrane Macromolecules", pp. 47-51, 1985, Biochem J., vol. 231.
McKerrow et al., "Schistosoma Mansoni: Cercarial Degradation of a Radioactively Labeled Collagen Gel", pp. 249-254, 1982, Exp. Parasitol., vol. 53.
McReynolds, et al., "A Large Cuticular Protein from D. Immitis that is Also an Excretory or Secretory Product," pp. 173-174, 1989, 38th Meeting, Am. J. Trop. Med. Hyg., Abstract #233, Dec.
McReynolds, et al., "Cloning of a Highly Repeated Protein Located in the Gut of Filarial Parasites, " p. 295, 1989, 38th Annual Meetin g, Am. J. Trop. Med. Hyg., Abstract #445, Dec.
Mok, et al., "Solubilization of Epicuticular Antigen from Dirofilaria Immitis Third-Stage Larvae", pp. 173-182, 1988, Mol. Biochem. Parasitol., vol. 31.
Noble, et al. "Phylum Nematoda", pp. 256-322, 1982 (See p. 256) Parasitol.: The Biology of Animal Parasites, Section V.
Parab, et al., "Characterization of a Monoclonal Antibody Against Infective Larvae of Brugia Malayi", pp. 169-174, 1988, J. Immunol., vol. 64.
Petralanda, et al. "Studies on a Filarial Antigen With Collagenase Activity", pp. 51-59, 1986 Mol. Biochem. Parasitol., vol. 19.
Phillipp, et al., "Biochemical and Immunologic Characterization of a Major Surface Antigen of Dirofilaria Immitis Infective Larvae", pp. pp. 2621-2627, 1986, J. Immunol., vol. 136(7), Apr.
Richer et al. "Dirofilaria Immitis: Proteases Produced By Third- And Fourth-Stage Larvae", pp. 213-222, 1992, Exp. Parasitol., vol. 75.
Robertson et al. "Toxocara Canis: Proteolytic Enzymes Secreted by the Infective Larvae in Vitro", pp. 30-36, 1989, Exp. Parasitol., vol. 69.
Rogers, "Enzymes in th

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