Nucleic acid molecules encoding modified polyketide...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S069100, C435S183000, C435S252350, C435S252300, C435S320100, C435S091400, C536S023100, C536S023200, C536S023700

Reexamination Certificate

active

07001747

ABSTRACT:
Nucleic acid molecules encoding at least part of a Type I polyketide synthase, and having a polylinker with multiple restriction enzyme sites in place of one or more PKS genes encoding enzymes associated with reduction, optionally further including nucleic acid incorporated into the polylinker, the further nucleic acid encoding one or more reductive enzymes; plasmids incorporating such nucleic acids; host cells transfected with such plasmids; methods relating thereto.

REFERENCES:
Elkmanns et al. A family ofCorynebacterium glutamicum/Escherichia colishuttle vectors for cloning, controlled gene expression, and promoter probing. Gene (1991) 102(1): 93-98.

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