Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2005-07-12
2005-07-12
Nelson, Amy J. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S290000, C435S320100, C435S468000, C536S023100, C536S023600
Reexamination Certificate
active
06916973
ABSTRACT:
The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which DNA sequences coding for “active site” amino acid residues have been mutated to generate hyperactive phytochromes. In particular; a serine/threonine residue at the hinge between the N- and C-terminal domains as well as at the N-terminal serine/threonine cluster of phytochromes (e.g., serine-598 and serine-7 in oat phytochrome A) for (a) Pr/Pfr-dependent phosphorylation and (b) dephosphorylation by a phytochrome phosphatase (PP2A) was substituted with alanine. (c) In addition, amino acid residues within the phytochrome chromophore pocket are mutated to generate the bathchromic shift of the Pr-absorption band of both wild type and above-mentioned mutant phytochromes. The plants with the bathchromically shifted absorption spectrum are expected to respond to the canopy and shade conditions for growth and greening responses to far-red light with greater efficiency than are the wild type plants with normal absorption band maxima. These mutative modifications confer hyperactivity to the far-red light responsive phytochromes A. Thus, the biological activity of the modified oat PHYA was shown to be hyperactive compared to wild type PHYA, characterized by its ability to reduce internode elongation of adult plants. Overexpression of the phytochrome phosphatase exhibits a suppressed growth with shorter internodes and belated flowering, qualitatively consistent with the phenotype of a ser598ala mutant oat phytochrome. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with reduced stature.
REFERENCES:
Casal et al (2002 Plant Physiology 129:1127-1137).
Hershey et al (1993, NCBI Accession No. X03242).
Bowie et al, Science 247:1306-1310, 1990.
McConnell et al, Nature 411 (6838):709-713, 2001.
Smith et al, Plant, Cell and Envir. 20, 1997, pp. 840-844, The shade avoidance syndrome: multiple responses mediated by.
Neff et al, Genes & Development, 14, 2000, pp. 257-271, Light: an indicator of time and place.
Yeh et al, Proc. Natl. Acad. Sci., vol. 95, pp. 13976-13981, Nov. 1998, Eukaryotic phytochromes: Light-regulated serine.
Lapko et al, Protein Science 8, 1999, pp. 1032-1044, Mass spectrometric characterization of oat phytochrome A: Isoforms.
Park et al, Cell & Devlopment Biol., vol. 11, 2000, pp. 449-456 Inter-domain crosstalk in the phytochrome molecules.
Lapko et al, Biochemistry 36, 1997, pp. 10595-10599, Post-translational Modification of Oat Phytochrome A:.
Bhoo et al, J. Am. Chem. Soc. 119, 1997, pp. 11717-11718, Phytochrome Photochromism Probed by Site-Directed Mutations.
Fankhauser et al, SCIENCE, vol. 284, May 28, 1999, pp. 1539-1541, PKS1, a Substrate Phosphorylated by Phytochrome That.
Quail et al, SCIENCE, vol. 268, May 5, 1995, pp. 675-680, Phyto-chromes: Photosensory Perception and Signal Transduction.
Shinomura et al, Proc. Natl. Acad. Sci, vol. 93, pp. 8129-8133 Action spectra for phytochrome A- and B-specific.
Boylan et al, Proc. Natl. Acad. Sci., vol. 88, pp. 10806-10810, Dec. 1991, Phytochrome A overexpression inhibits hypocotyl.
Kim Jeong Il
Park Chung Mo
Soh Moon Soo
Song Pill Soon
Yang Ki Young
Baum Stuart F.
Jacobson & Holman PLLC
Korea Kumho Petrochemical Co. Ltd.
Nelson Amy J.
LandOfFree
Nucleic acid molecules encoding hyperactive mutant... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nucleic acid molecules encoding hyperactive mutant..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid molecules encoding hyperactive mutant... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3415767