Nucleic acid molecules encoding enzymes having fructosyl...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S193000, C536S023200

Reexamination Certificate

active

07153674

ABSTRACT:
Described are nucleic acid molecules encoding enzymes having fructosyl polymerase activity. These enzymes are sucrose dependent sucrose fructosyltransferases (SST) enzymes. Furthermore, vectors and host cells are described containing the nucleic acid molecules, in particular transformed plant cells and plants that can be regenerated from them and that express the described SSTs. Furthermore, methods for the production of short-chain fructosyl polymers using the described hosts and/or the SSTs produced by them are described.

REFERENCES:
patent: WO 94/14970 (1994-07-01), None
patent: WO 95/13389 (1995-05-01), None
patent: WO 96/01904 (1996-01-01), None
patent: WO 96/21023 (1996-07-01), None
patent: WO 97/29186 (1997-08-01), None
Elke M. Hellwege, et al., “Differences in Chain Length Distribution of Inulin fromCynara scolymusandHelianthus tuberosusare Reflected in a Transient Plant Expression System Using the Respective 1-FFT cDNAs,”FEBS Letters, 427, pp. 25-28 (1998).
Elke M. Hellwege et al., “Transgenic Potato Tubers Accumulate High Levels of 1-Kestose and Nystose: Functional Identification of a Sucrose 1-Fructosyltransferase of Artichoke (Cynara scolymus) Blossom Discs,”The Plant Journal, 12(5), pp. 1057-1065 (1997).
Norbert Sprenger et al., “Fructan Synthesis in Transgenic Tobacco and Chicory Plants Expressing Barley Sucrose: Fructan 6-Fructosyltransferase,”FEBS Letters, 400, pp. 355-358 (1997).
I. Vijn et al., “Fructan of the Inulin Neoseries is Synthesized in Transgenic Chicory Plants (Cichorium intybusL.) Harbouring Onion (Allium cepaL.) Fructan:Fructan 6G-Fructosyltransferase,”The Plant Journal, 11(3), pp. 387-398 (1997).
Hellwege et al., Gene Bank, Accession No. Y09662 (Oct. 1997).

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