Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-08-06
2009-06-30
Huynh, Phuong (Department: 1644)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S006120, C435S007210, C435S007250, C435S007940, C435S332000, C436S517000, C436S520000, C536S023500
Reexamination Certificate
active
07553629
ABSTRACT:
The present invention relates to novel nucleic acid molecules encoding a Rhesus D antigen contributing to the weak D phenotype which are characterized by one or a combination of missense mutations or by a gene conversion involving exons 6 to 9 of the RHD and RHCE genes. The present invention further relates to vectors comprising the nucleic acid molecules of the invention, to hosts transformed with said vectors, to proteins encoded by said nucleic acid molecules and to methods of producing such polypeptides. The fact that missense mutations and the conversion referred to above can be directly correlated to the weak D phenotype has a significant impact on the routine testing of blood samples. For example, oligonucleotides and antibodies can now be designed that generally allow the detection of weak D phenotypes in a sample. Such oligonucleotides, antibodies as well as a variety of diagnostic methods all fall within the scope of the present invention. RhD antigens encoded by the novel nucleic acid molecules may be used for the characterization, standardization and quality control of monoclonal and polyclonal anti-D antisera. Finally, the invention relates to a kit useful for testing for the presence of weak D phenotypes.
REFERENCES:
patent: 5665356 (1997-09-01), De Burgh Bradley et al.
patent: 6200802 (2001-03-01), Greene et al.
Singleton et al, Blood 95: 12-18, 2000.
Simsek et al, Blood 85(10): 2975-2980, May 15, 1995.
Makrigioros et al, Human Mutation 23: 406-412, 2004.
Avent, N.D., “The Rhesus Blood Group System: Insights From Recent Advances in Molecular Biology”, Transfusion Medicine Reviews, 13:245-266 (1999).
Avent, N.D., et al., Evidence of Genetic Diversity Underlying RH D-, Weak D (D″), and Partial D Phenotypes as Determined by Muultiplex Polymerase Chain Reaction Analysis of theRHDGene, Blood, 89:2568-2577 (1997).
Avent, N.D., et al., “The Rh Blood Group System: a Review”, Blood, 95:375-387 (2000).
Cherif-Zahar, B., et al., “Molecular Cloning and Protein Structure of a Human Blood Group Rh Polypeptide”, Proc. Natl. Acad. Sci. USA, 87:6243-6247 (1990).
Jones J.W., et al., “Quantitation of Rh D Antigen Sites on Weak D and D Variant Red Cells by Flow Cytometry”, Vox Sang 71:176-183 (1996).
Legler, T.J., et al., “RHD Genotyping in Weak D Phenotypes by Multiple Polymerase Chain Reactions”, Transfusion 38:434-440 (1998).
Maas, J.H., et al., “Rh-D-Genotypisierung auf Exon 2, 5 und 7 von Deutschen und Papaneschen Blutspendern Mit Sequenzspezifischer Polymerasekettenreaktion”, Transfusionamedizin, 34:203-209 (1997).
Makrigiorgos, PCR-Based Detection of Minority Point Mutations, Human Mutation 23:406-412 (2004).
Ngo, T.J., et al., “Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox”, The Protein Problem and Tertiary Structure Prediction, Birkhauser Boston, pp. 492-495 (1994).
Rouillac, C., et al., “Leu110Pro Substitution in the RhD Polypeptide Is Responsible for the DVIICategory Blood Group Phenotype”, American Journal of Hematology 49:87-88 (1995).
Rouillac, C., et al., “Molecular Basis of the Altered Antigenic Expression of RhD in Weak D (D″) and RhC/e in RNPhenotypes”, Blood, 87(11):4853-4861 (1996).
Salvignol, I., et al., Biochem. Genet 32:201-221 (1994).
Sambrook, J., et al., Molecular Cloning, 1989, Cold Spring Harbor Laboratory, CSH, NY, Ch. 11 & 17.
Simsek, S., et al., Rapid RH D Genotyping by Polymerase Chain Reaction-Based Amplification of DNA, Blood, 85(10):29750-2980 (1995).
Singleton, B.K., et al., The Presence of an RHD Pseudogene Containing a 37 Base Pair Duplication and a Nonsense Mutation in Africans with the Rh D-Negative Blood Group Phenotype, Blood, 95(1):12-18 (2000).
Skolnick, J., et al., “From Genes to Protein Structure and Function: Novel Applications of Computational Approaches in the Genomic Era”, Tiptech, 18:34-36 (2000).
Stryer, L, et al., Biochemistry, Third Edition, W.H. Freeman Company, New York, pp. 31-33 (1998).
Wagner, F.F., et al., “Molecular Basis of Weak D Phenotypes”, Blood, 93:385-393 (1999).
Wagner, FF, “Influence of Rh Phenotype on the Antigen Density of C, c and D: Flow Cytometric Study Using a Frozen Standard Red Cell”, Transfusion, 34:671-676 (1994).
Wallace, R.B., et al., Oligonucleotide Probes for the Screening of Recombinant DNA Libraries, Methods Enzymol. 152:432-439 (1987).
Westhoff, C.M., et al., Blood 83:3098-3100 (1994).
Flegel Willy A.
Wagner Franz F.
Flegel Willy A.
Huynh Phuong
Pillsbury Winthrop Shaw & Pittman LLP
LandOfFree
Nucleic acid molecules correlated with the Rhesus weak D... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Nucleic acid molecules correlated with the Rhesus weak D..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid molecules correlated with the Rhesus weak D... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4141081