Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Patent
1998-11-03
2000-07-25
Carlson, Karen Cochrane
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
435 691, 4353201, 435325, 4352523, 536 232, C12N 900, C12P 2106, C07H 1700
Patent
active
06093560&
ABSTRACT:
A SOK polypeptide, an isolated DNA having a nucleotide sequence encoding a SOK polypeptide, and a method of determining whether a candidate compound modulates SOK-1 activity or expression, comprising the steps of providing a first and a second recombinant cell expressing a SOK gene; introducing a candidate compound into the first cell, but not into the second cell; measuring a SOK function in the first and second cells; and comparing the results obtained with the first and second SOK transformed cells, wherein an increase or decrease in the SOK function in the first cell compared to the second cell is an indication that the candidate compound modulates SOK expression or activity.
REFERENCES:
patent: 5830699 (1998-11-01), Force et al.
Ansorge, W. et al., "Performance of an automated system for capillary microinjection into living cells," Journal of Biochemical and Biophysical Methods, 16:283-292 (1988).
Brown, D. et al., "Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS)," Histochem. Cell Biol. 105:261-267 (1996).
Bruder, J.T. et al., "Serum-, TPA-, and Ras-induced expression from AP-1/Ets-driven promoters requires Raf-1 Kinase," Genes & Development 6:545-556 (1992).
Chen J., et al., "Regulation of Protein Serine-Threonine Phosphatase Type-2A by Tyrosine Phosphorylation," Science 257:1261-1264 (1992).
Creasy, C.L. et al., "Cloning and Characterization of a Human Protein Kinase with Homology to Ste20," J. Biol. Chem. 270:21695-21700 (1995).
van Dam, H. et al., "AFT-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotixoc agents," EMBO J. 14:1798-1811 (1995).
Friesen, H. et al., "Mutation of the SPS1-encoded protein kinase of Saccharomyces cerevisiae Leads to defects in transcription and morphology during spore formation," Genes & Development 8:2162-2175 (1994).
Galcheva-Gargova, Z. et al., "An Osmosensing Signal Transduction Pathway in Mammalian Cells," Science 265:806-808 (1994).
Guan, K. et al., "Eukaryotic Proteins Expressed in Escherichia coli: An Improved Thrombin Cleavage and Purification Procedure of Fusion Proteins with Glutathione S-transferase," Anal. Biochem. 192:262-267 (1991).
Han, J. et al., "A MAP Kinase Targeted by Endotoxin and Hyperosmolarity in Mammalian Cells," Science 265:808-811 (1994).
Hanks et al., Protein Kinase Catalytic Domain Sequence Database: Identification of Conserved Features of Primary Structure and Classification of Family Members, Methods in Enzymology, vol. 200, 38-62, 1991.
Herskowitz, I., "MAP Kinase Pathways in Yeast: For Mating and More," Cell 80:187-197.
Hibi, M. et al., "Identification of an oncoprotein- and UV-responseive protein kinase that binds and potentiates the c-June activation domain," Genes & Development 7:2135-2148 (1993).
Katz, P. et al., "Differential Expression of a Novel Protein Kinase in Human B Lymphocytes," J. Biol. chem. 269:16802-16809 (1994).
Kharbanda, S. et al., "Ionizing Radiation Stimulates a Grb2-mediated Association of the Stress-activated Protein Kinase with Phosphatidylinositol 3-Kinase," J. Biol. chem. 270:18871-18874 (1995).
Kharbanda, S. et al., "Activation of the c-Abl tyrosine kinase in the stress resonse ot DNA-damaging agents," Nature 376:785-788 (1995).
Krisak, L. et al., "SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae," Genes & Development 8:2151-2161 (1994).
Leberer, E. et al., "The protein kinase homologue ste20p is required to link the yeast pheromone response G-protein .beta..gamma. subunits to downstream signaling components," EMBO J. 11:4815-4824 (1992).
Livingstone, C. et al., "ATF-2 contains a phosphorylation-dependant transcriptional activation domain," EMBO J. 14;1785-1797 (1995).
Manser, E. et al., "A brain serine/threonine protein kinase activated by Cdc42 and Racl," Nature 367:40-45 (1994).
Martin, G. A., "A Novel serine kinase activated by racl/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20," EMBO J. 14:1970-1978 (1995).
Morooka, H. et al., "Ischemia and Reperfusion Enhance ATF-2 and c-jun Binding to cAMP Response Elements and to an AP-1 Binding Site from the c-jun Promoter," J. Biol. Chem. 270:30084-30092 (1995).
Osada, S. et al., "YSK1, a novel mammalian protein kinase structurally related to Ste20 and SPS1, but is not involved in the known MAPK pathways," Oncogene 14:2047-2057 (1997).
Pagano, M. et al., "Cyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts," Genes & Development 8:1627-1639 (1994).
Polverino, A. et al., "Activation of Mitogen-activated Protein Kinase Cascades by p21-activated protein Kinases in Cell-free Extracts of Xenopus Oocytes," J. Biol. Chem. 270:26067-26070 (1995).
Pombo, C. M. et al., "The Stress-activated Protein Kinases Are Major c-Jun Amino-terminal Kinases Activated by Ischemia and Reperfusion," J. Biol. Chem. 269:26546-26551 (1994).
Pombo, C. M. et al., "Activation of the SAPK pathway by the human STE20 homologue germinal centre kinase," Nature 377:750-754 (1995).
Pombo, C. M. et al., "Activation of a human Ste20-like kinase by oxidant stress defines a novel stress response pathway," EMBO J. 15:4537-4546 (1996).
Radler-Pohl, A. et al., "UV-induced activation of AP-1 involves obligatory extranuclear steps including Raf-1 kinase," EMBO J. 12:1005-1012 (1993).
Rouse, J. et al., "A Novel Kinase Cascade Triggered by Stress and Heat Shock That Stimulates MAPKAP Kinase-2 and Phosphorylation of the Small Heat Shock Proteins," Cell 78:1027-1037 (1994).
Russo, T. et al., "A p53-independent Pathway for Activation of WAF1/CIP1 Expression Following Oxidative Stress," J. Biol. Chem. 270:29386-29391 (1995).
Sanches, I. et al., "Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun," Nature 372:794-798 (1994).
Verma, I.M. et al., "Rel/Nf-IkB family: intimate tales of association and dissociation," Genes & Development 9:2723-2735 (1995).
Witzgall, R. et al., Kid-1, a Putative Renal Transcription Factor: Regulation during Ontogeny and in Response to Ischemia and Toxic Injury, Mol. Cell. Biol. 13:1933-1942 (1993).
Wu et al., Molecular Characterization of Ste20p, a Potential Mitogen-activated Protein or Extracellular Signal-regulated Kinase Kinase (MEK) Kinase Kinase from Saccharomyces cerevisiae, The Journal of Biological Chemistry, vol. 270, No. 27, 15984-15993, 19945.
Zhou, G. et al., "Components of a New Human Protein Kinase Signal Transduction Pathway," J. Biol. Chem. 270:12665-12669 (1995).
Bonventre Joseph
Force Thomas
Kyriakis John M.
Pombo Celia M.
Carlson Karen Cochrane
The General Hospital Corporation
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