Nucleic acid melting analysis with saturation dyes

Details Nucleic acid melting analysis with saturation dyes Nucleic acid melting analysis with saturation dyes

2006-07-13

2008-11-25

Anderson, Rebecca L (Department: 4131)

Organic compounds -- part of the class 532-570 series

Organic compounds

Four or more ring nitrogens in the bicyclo ring system

C544S245000, C544S334000, C548S152000

Reexamination Certificate

active

07456281

ABSTRACT:
Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

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