Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1989-04-20
1990-05-01
Brown, Johnnie R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 28, 536 29, 536127, 435803, C07H 2100, C07H 2102, C07H 2104
Patent
active
049219529
ABSTRACT:
A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain with the DNA. These products can be removed with organic extraction, but the process steps associated with organic extraction reduce the size of DNA fragments available for experimental use. The present process removes the waste products by dialyzing a solution containing the DNA against a solution containing polyethylene glycol (PEG). The waste products dialyze into the PEG leaving isolated DNA. The remaining DNA has been prepared with fragments containing more than 160 kb. The isolated DNA has been used in conventional protocols without affect on the protocol.
REFERENCES:
Manintis et al., "Molecular Cloning, Abstrating Manual", Cold Spring Nebraska Laboratory, 1982, pp. 80-83.
Maniatis et al., "Molecular Cloning, a Laboratory Manual", Cold Spring Harbor laboratory, 1982, pp. 93-94.
Schlieg et al., "Practical Methods in Molecular Biology", Spring Verlag, New York, 1981, pp. 64-65.
Longmier et al., Nucleic Acids Research, vol. 15(2), pp. 859-860 (1987).
J. L. Longmire et al., "A Rapid and Simple Method for the Isolation of High Molecular Weight Cellular and Chromosome-Specific DNA in Solution Without the use of Organic Solvents", Nucleic Acids Research, 15, No. 2, p. 859 (1987).
Hildebrand Carl E.
Lewis Annette K.
Longmire Jonathan L.
Brown Johnnie R.
Crane L. Eric
Gaetjens Paul D.
Moser William R.
The United States of America as represented by the United States
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